Cytokine mRNA expression in mouse colon: IL-15 mRNA is overexpressed and is highly sensitive to a fibre-like dietary component (short-chain fructo-oligosaccharides) in an Apc gene manner.

Abstract

On the basis of studies using the Min mouse model of colon carcinogenesis, we have recently proposed that a fibre-like food (short-chain fructo-oligosaccharides, sc-FOS) fermented in the colon may stimulate a mechanism of cancer immunosurveillance. In the present paper, we have investigated the expression of cytokines as potential effector molecules. Interleukin (IL-)4, IL-5, IL-13, IL-15 and interferon (INF)-gamma mRNAs were detected by a multi-probe ribonuclease protection assay in C57BL/6J and Min mouse colons. IL-15 mRNA expression was significantly amplified (P=0.01) by the sc-FOS-enriched diet in the colon of Min mice.

• Authors: E Bassonga, V Forest, F Pierre, F Bornet, P Perrin, K Meflah, J Menanteau
• Journal: Cytokine (Cytokine) Vol. 14 Issue 4 Pg. 243-6 (May 21 2001) ISSN: 1043-4666 [Print] England
• PMID: 11448126 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 Academic Press.

Chemical References

• Cytokines
• Dietary Fiber
• Interleukin-15
• Oligosaccharides
• RNA, Messenger
• fructooligosaccharide

Topics

• Animals
• Colon (immunology, metabolism)
• Cytokines (biosynthesis)
• Diet
• Dietary Fiber (administration & dosage)
• Genes, APC
• Interleukin-15 (biosynthesis, genetics)
• Mice
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Oligosaccharides (administration & dosage)
• RNA, Messenger (biosynthesis)