Abstract | BACKGROUND: OBJECTIVE: The aim of this study is to prepare and preclinically evaluate a hybrid peptide comprising 7 T-cell determinants of Cry j 1 and Cry j 2, the major Japanese cedar pollen allergens. METHODS: The recombinant hybrid peptide was prepared after immunodominance of 7 T-cell determinants was confirmed by means of PBMC proliferation assay in 113 volunteers with pollinosis. The hybrid peptide was compared with a mixture of the 7 T-cell determinants in a dose-dependent PBMC proliferation assay in 6 volunteers with pollinosis. PBMC proliferation and binding activity of serum IgE antibody against the hybrid peptide, Cry j 1, and Cry j 2 were investigated in 48 volunteers with pollinosis. RESULTS: The hybrid peptide induced T-cell proliferation with an average 100-fold lower concentration than a mixture of the 7 peptides. PBMCs from 44 (92%) of 48 volunteers proliferated against the hybrid peptide, with significant correlation (r = 0.87) in T-cell proliferation against Cry j 1 and Cry j 2. No serum IgE antibodies specific to Cry j 1 or Cry j 2 bound to the hybrid peptide. CONCLUSION: A hybrid peptide comprising 7 T-cell determinants has the potential for inducing T-cell proliferative responses that is superior to the potential of a mixture of the T-cell determinants and comparable with that of Cry j 1 and Cry j 2. The hybrid peptide will be of use in specific immunotherapy against Japanese cedar pollinosis.
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Authors | K Hirahara, T Tatsuta, T Takatori, M Ohtsuki, H Kirinaka, J Kawaguchi, N Serizawa, Y Taniguchi, S Saito, M Sakaguchi, S Inouye, A Shiraishi |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 108
Issue 1
Pg. 94-100
(Jul 2001)
ISSN: 0091-6749 [Print] United States |
PMID | 11447388
(Publication Type: Journal Article)
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Chemical References |
- Allergens
- Antigens, Plant
- Cry j I protein, Cryptomeria japonica
- Cry j II protein, Cryptomeria japonica
- Epitopes, T-Lymphocyte
- Peptides
- Plant Proteins
- Recombinant Fusion Proteins
- Immunoglobulin E
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Topics |
- Adult
- Allergens
(genetics, immunology)
- Amino Acid Sequence
- Antigens, Plant
- Cells, Cultured
- Desensitization, Immunologic
- Drug Evaluation, Preclinical
- Epitopes, T-Lymphocyte
(immunology)
- Female
- Humans
- Immunoglobulin E
(immunology)
- Lymphocyte Activation
- Male
- Molecular Sequence Data
- Peptides
(genetics, immunology)
- Plant Proteins
(genetics, immunology)
- Pollen
(genetics, immunology)
- Recombinant Fusion Proteins
(immunology)
- Rhinitis, Allergic, Seasonal
(immunology, therapy)
- T-Lymphocytes
(immunology)
- Trees
(immunology)
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