Abstract |
Bone morphogenetic proteins are members of the transforming growth factor-beta superfamily. They are widely expressed in the mammalian nervous system, where they exert trophic effects on several neuronal populations. We studied the neurotrophic activity of bone morphogenetic protein-2 and bone morphogenetic protein-7 (also called osteogenic protein-1) on cultured striatal cells, previously shown to express bone morphogenetic protein ligands and receptors. Our results indicate that only bone morphogenetic protein-2 promoted the differentiation of GABAergic neurons, especially of the calbindin-positive subpopulation, the subset of projecting striatal neurons that degenerates in Huntington's disease. Bone morphogenetic protein-2 increased the area, perimeter and degree of arborization of GABAergic neurons, promoting calbindin phenotype without altering proliferation or apoptosis. In contrast, neither bone morphogenetic protein-2 nor -7 affected striatal cholinergic interneurons. However, they both increased the number of glial fibrillary acidic protein-positive cells. Suppression of glial proliferation with 5-fluorodeoxyuridine did not abolish bone morphogenetic protein-2 effects on the differentiation of striatal neurons, ruling out an indirect mechanism through astrocytes. In conclusion, our results show that bone morphogenetic protein-2 promotes the differentiation of cultured GABAergic striatal neurons, suggesting that bone morphogenetic proteins are involved in the development of the striatum.
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Authors | E Gratacòs, N Checa, J Alberch |
Journal | Neuroscience
(Neuroscience)
Vol. 104
Issue 3
Pg. 783-90
( 2001)
ISSN: 0306-4522 [Print] United States |
PMID | 11440809
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites, Antineoplastic
- Bmp2 protein, rat
- Bmp7 protein, rat
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Protein 7
- Bone Morphogenetic Proteins
- Calbindins
- S100 Calcium Binding Protein G
- Transforming Growth Factor beta
- Floxuridine
- gamma-Aminobutyric Acid
- Choline O-Acetyltransferase
- Uridine
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Topics |
- Animals
- Antimetabolites, Antineoplastic
(pharmacology)
- Astrocytes
(cytology, drug effects, metabolism)
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Protein 7
- Bone Morphogenetic Proteins
(pharmacology)
- Calbindins
- Cell Count
- Cell Differentiation
(drug effects, physiology)
- Cell Division
(drug effects, physiology)
- Cell Size
(drug effects, physiology)
- Cell Survival
(drug effects, physiology)
- Cells, Cultured
(cytology, drug effects, metabolism)
- Choline O-Acetyltransferase
(metabolism)
- Dendrites
(drug effects, metabolism, ultrastructure)
- Dose-Response Relationship, Drug
- Drug Administration Schedule
- Fetus
- Floxuridine
(pharmacology)
- Immunohistochemistry
- Neostriatum
(cytology, drug effects, embryology)
- Phenotype
- Rats
- Rats, Sprague-Dawley
- S100 Calcium Binding Protein G
(drug effects, metabolism)
- Transforming Growth Factor beta
- Uridine
(pharmacology)
- gamma-Aminobutyric Acid
(metabolism, pharmacokinetics)
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