Bone morphogenetic protein-2, but not bone morphogenetic protein-7, promotes dendritic growth and calbindin phenotype in cultured rat striatal neurons.

Abstract

Bone morphogenetic proteins are members of the transforming growth factor-beta superfamily. They are widely expressed in the mammalian nervous system, where they exert trophic effects on several neuronal populations. We studied the neurotrophic activity of bone morphogenetic protein-2 and bone morphogenetic protein-7 (also called osteogenic protein-1) on cultured striatal cells, previously shown to express bone morphogenetic protein ligands and receptors. Our results indicate that only bone morphogenetic protein-2 promoted the differentiation of GABAergic neurons, especially of the calbindin-positive subpopulation, the subset of projecting striatal neurons that degenerates in Huntington's disease. Bone morphogenetic protein-2 increased the area, perimeter and degree of arborization of GABAergic neurons, promoting calbindin phenotype without altering proliferation or apoptosis. In contrast, neither bone morphogenetic protein-2 nor -7 affected striatal cholinergic interneurons. However, they both increased the number of glial fibrillary acidic protein-positive cells. Suppression of glial proliferation with 5-fluorodeoxyuridine did not abolish bone morphogenetic protein-2 effects on the differentiation of striatal neurons, ruling out an indirect mechanism through astrocytes. In conclusion, our results show that bone morphogenetic protein-2 promotes the differentiation of cultured GABAergic striatal neurons, suggesting that bone morphogenetic proteins are involved in the development of the striatum.

• Authors: E Gratacòs, N Checa, J Alberch
• Journal: Neuroscience (Neuroscience) Vol. 104 Issue 3 Pg. 783-90 ( 2001) ISSN: 0306-4522 [Print] United States
• PMID: 11440809 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimetabolites, Antineoplastic
• Bmp2 protein, rat
• Bmp7 protein, rat
• Bone Morphogenetic Protein 2
• Bone Morphogenetic Protein 7
• Bone Morphogenetic Proteins
• Calbindins
• S100 Calcium Binding Protein G
• Transforming Growth Factor beta
• Floxuridine
• gamma-Aminobutyric Acid
• Choline O-Acetyltransferase
• Uridine

Topics

• Animals
• Antimetabolites, Antineoplastic (pharmacology)
• Astrocytes (cytology, drug effects, metabolism)
• Bone Morphogenetic Protein 2
• Bone Morphogenetic Protein 7
• Bone Morphogenetic Proteins (pharmacology)
• Calbindins
• Cell Count
• Cell Differentiation (drug effects, physiology)
• Cell Division (drug effects, physiology)
• Cell Size (drug effects, physiology)
• Cell Survival (drug effects, physiology)
• Cells, Cultured (cytology, drug effects, metabolism)
• Choline O-Acetyltransferase (metabolism)
• Dendrites (drug effects, metabolism, ultrastructure)
• Dose-Response Relationship, Drug
• Drug Administration Schedule
• Fetus
• Floxuridine (pharmacology)
• Immunohistochemistry
• Neostriatum (cytology, drug effects, embryology)
• Phenotype
• Rats
• Rats, Sprague-Dawley
• S100 Calcium Binding Protein G (drug effects, metabolism)
• Transforming Growth Factor beta
• Uridine (pharmacology)
• gamma-Aminobutyric Acid (metabolism, pharmacokinetics)