cDNA cloning, chromosome assignment, and genomic structure of a human gene encoding a novel member of the RBM family.

Abstract

We have cloned and characterised a novel human gene mapping to chromosome 20q11.2. A partial transcript was initially isolated from a human cDNA library transcribed from RNA of the colon carcinoma cell line T-84. In order to determine the full coding sequence of this novel mRNA, we isolated seven cDNA clones from a human cDNA library transcribed from RNA of the acute monocytic leukemia cell line THP1 by colony hybridization. On Northern blot analysis of four human cell lines, the cDNAs isolated hybridize with an abundantly expressed mRNA species of 3.5 kb. A full-length cDNA transcript of this novel mRNA has an open reading frame of 2,796 bp encoding a protein with a calculated molecular weight of 97 kDa. Two repetitive structural consensus motifs are contained within the deduced protein sequence, namely five distinct RNA binding motifs and two proline rich regions. The derived protein sequence also contains putative transmembrane domains. These structural motifs identify this novel protein as a member of an expanding protein family containing RNA binding motifs (RBM). As seen from recently completed sequence of the genomic area encoding this novel mRNA by the Sanger Centre Human Genome Project, the coding region of this gene, RBM12, is intronless.

• Authors: C Stover, G Gradl, I Jentsch, M R Speicher, R Wieser, W Schwaeble
• Journal: Cytogenetics and cell genetics (Cytogenet Cell Genet) Vol. 92 Issue 3-4 Pg. 225-30 ( 2001) ISSN: 0301-0171 [Print] Switzerland
• PMID: 11435693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 S. Karger AG, Basel.

Chemical References

• DNA, Complementary
• Nuclear Proteins
• RBM12 protein, human
• RBMY1A1 protein, human
• RNA, Messenger
• RNA-Binding Proteins

Topics

• Amino Acid Motifs
• Amino Acid Sequence
• Base Sequence
• Blotting, Southern
• Chromosomes, Human, Pair 20 (genetics)
• Cloning, Molecular
• DNA, Complementary (genetics)
• Exons (genetics)
• Gene Library
• Human Genome Project
• Humans
• In Situ Hybridization, Fluorescence
• Introns (genetics)
• Male
• Molecular Sequence Data
• Molecular Weight
• Multigene Family (genetics)
• Nuclear Proteins
• Open Reading Frames (genetics)
• Physical Chromosome Mapping
• Protein Biosynthesis
• Protein Structure, Tertiary
• RNA, Messenger (analysis, genetics)
• RNA-Binding Proteins (chemistry, genetics)
• Tumor Cells, Cultured