Abstract | OBJECTIVE: To study the effect of benzene on aneupoidy of oocytes and female pronucleus of one cell zygotes. METHOD: The NIH adult female mice were treated with varies doses of benzene (942, 1,881 and 3,762 mg/kg respectively) via single gavage and (706, 1,922 and 4,864 mg/m3, respectively) multiple inhalation. After gavage, the mice were pair-matched (1:1) with males overnight. The ovulated oocytes and the one cell zygotes were collected for cytogenetic analysis and the frequencies of aneuploidy were detected. RESULTS: The frequencies of aneuploid in M II oocytes significantly increased in the three groups treated with inhalation (7.06%, 7.50% and 7.76%, respectively, control group 0.00%, P < 0.05) of benzene, as compared with those in the control group (1.30%), with a dose-dependent response. Meiotic delay of M I oocytes was observed, with the frequency of M I oocytes of 1.16%, 3.61% and 5.75%, respectively, P < 0.05, in a dose-dependent response, too. In gavage treatment group, increase in frequency of M II oocyte aneuploidy was only observed in the high-dose group, and aneupliody frequency of female pronucleus in one cell zygotes was not induced. CONCLUSION: Exposure to high-dose benzene via gavage or inhalation was able to induce increase in aneuploidy frequency of M II oocytes in mice.
|
Authors | Q Zeng, L Zheng, L Deng |
Journal | Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
(Zhonghua Yu Fang Yi Xue Za Zhi)
Vol. 35
Issue 2
Pg. 87-9
(Mar 2001)
ISSN: 0253-9624 [Print] China |
PMID | 11413689
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Aneuploidy
- Animals
- Benzene
(toxicity)
- Female
- Mice
- Oocytes
(drug effects, pathology)
- Zygote
(drug effects, pathology)
|