Comparison of PCR and common clinical tests for the diagnosis of H. pylori in dyspeptic patients.
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| Abstract |
Helicobacter pylori has been recognized as a major gastric pathogen. The objective of this study was to assess the diagnostic value of common clinical tests to detect H. pylori infection, by comparison with PCR. Serum and gastric biopsy specimens from 106 dyspeptic patients were examined. Serology was performed with Pyloriset Dry test, and biopsies were examined histologically, for rapid urease activity and PCR amplification of an ureA gene segment of H. pylori. PCR primers were specific for H. pylori and required at least 1.47 pg of H. pylori DNA, corresponding to about 800 bacterial cells. According to serology, histology, rapid urease, and PCR, positive results were respectively found in 56%, 86%, 64%, and 85% of dyspeptic patients, primarily with gastritis. Relative to PCR, the sensitivity (and specificity) was 55% (38%) for serology, 86% (13%) for histology, 70% (69%) for urease. When combining histology and urease, Bayesian analysis of data indicated no advantage of using combined methods over rapid urease test alone. Histology should not any longer be considered a gold standard test for Helicobacter pylori. Urea breath test still seems the first option for non invasive diagnostic. If an invasive diagnostic is justified, highly specific and sensitive molecular methods should be used to examine specimens.
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| Authors | N Pacheco, V Mago, I Gómez, P Gueneau, M Guelrud, N Reyes, L R Pericchi, M G Domínguez-Bello |
| Journal | Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 39 Issue 4 Pg. 207-10 (Apr 2001) ISSN: 0732-8893 [Print] United States |
| PMID | 11404061 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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