Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.

The Tg.AC mouse carrying the v-Ha-ras structural gene is a useful model for the study of chemical carcinogens, especially those acting via non-genotoxic mechanisms. This study evaluated the efficacy of the non-toxic, water-soluble antioxidant from spinach, natural antioxidant (NAO), in reducing skin papilloma induction in female hemizygous Tg.AC mice treated dermally five times over 2.5 weeks with 2.5 microg 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-only group was considered as a control; the other two groups received, additionally, NAO topically (2 mg) or orally (100 mg/kg), 5 days/week for 5 weeks. Papilloma counts made macroscopically during the clinical observations showed a significant decrease in multiplicity (P<0.01) in the NAO topically treated group. According to histological criteria, papilloma multiplicity were lower in both topical-NAO and oral-NAO groups, but significantly so only in the oral-NAO mice (P<0.01). The beneficial effect of NAO in the Tg.AC mouse is reported.
AuthorsA Nyska, L Lomnitski, J Spalding, D B Dunson, T L Goldsworthy, V Ben-Shaul, S Grossman, M Bergman, G Boorman
JournalToxicology letters (Toxicol Lett) Vol. 122 Issue 1 Pg. 33-44 (May 31 2001) ISSN: 0378-4274 [Print] Netherlands
PMID11397555 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antioxidants
  • Carcinogens
  • Plant Extracts
  • Tetradecanoylphorbol Acetate
  • Administration, Cutaneous
  • Administration, Oral
  • Animals
  • Antioxidants (pharmacology)
  • Body Weight (drug effects)
  • Carcinogens (adverse effects)
  • Disease Models, Animal
  • Female
  • Genes, ras (genetics)
  • Genotype
  • Mice
  • Mice, Transgenic
  • Papilloma (chemically induced, pathology, prevention & control)
  • Plant Extracts (pharmacology)
  • Skin Neoplasms (chemically induced, pathology, prevention & control)
  • Spinacia oleracea (chemistry)
  • Survival Analysis
  • Tetradecanoylphorbol Acetate (adverse effects)

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