Abstract |
The Tg.AC mouse carrying the v-Ha-ras structural gene is a useful model for the study of chemical carcinogens, especially those acting via non-genotoxic mechanisms. This study evaluated the efficacy of the non-toxic, water-soluble antioxidant from spinach, natural antioxidant ( NAO), in reducing skin papilloma induction in female hemizygous Tg.AC mice treated dermally five times over 2.5 weeks with 2.5 microg 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-only group was considered as a control; the other two groups received, additionally, NAO topically (2 mg) or orally (100 mg/kg), 5 days/week for 5 weeks. Papilloma counts made macroscopically during the clinical observations showed a significant decrease in multiplicity (P<0.01) in the NAO topically treated group. According to histological criteria, papilloma multiplicity were lower in both topical- NAO and oral- NAO groups, but significantly so only in the oral- NAO mice (P<0.01). The beneficial effect of NAO in the Tg.AC mouse is reported.
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Authors | A Nyska, L Lomnitski, J Spalding, D B Dunson, T L Goldsworthy, V Ben-Shaul, S Grossman, M Bergman, G Boorman |
Journal | Toxicology letters
(Toxicol Lett)
Vol. 122
Issue 1
Pg. 33-44
(May 31 2001)
ISSN: 0378-4274 [Print] Netherlands |
PMID | 11397555
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antioxidants
- Carcinogens
- Plant Extracts
- Tetradecanoylphorbol Acetate
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Topics |
- Administration, Cutaneous
- Administration, Oral
- Animals
- Antioxidants
(pharmacology)
- Body Weight
(drug effects)
- Carcinogens
(adverse effects)
- Disease Models, Animal
- Female
- Genes, ras
(genetics)
- Genotype
- Mice
- Mice, Transgenic
- Papilloma
(chemically induced, pathology, prevention & control)
- Plant Extracts
(pharmacology)
- Skin Neoplasms
(chemically induced, pathology, prevention & control)
- Spinacia oleracea
(chemistry)
- Survival Analysis
- Tetradecanoylphorbol Acetate
(adverse effects)
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