Suppression of Ehrlich subcutaneous solid tumor growth by immunization with ganglioside GT1b of its origin, its IgM antibody or anti-idiotype antibody.

Abstract

Mice vaccinated with purified Ehrlich tumor-plasma GT1b admixed with Freund's adjuvant suppressed subcutaneous growth of the same tumor cells inoculated in vivo by 37.9%. The suppression was dose-dependent and was a result of humoral immune response against GT1b. Further, when mice were injected with varying doses of rabbit polyclonal anti-GT1b IgM antibody and challenged with Ehrlich tumor cells subcutaneously, a significant reduction in tumor growth (47.3%) was observed. Again, the suppression was dose-dependent. To strengthen furthier the observed therapeutic potential of this immunogenic GT1b, mice were immunized with anti-idiotype antibodies to GT1b raised in female Sprague Dawley rats by immunization with rabbit anti-GT1b IgM, which is expected to carry the structural image of GT1b. Immunization of mice with this anti-idiotype antibody was observed to suppress Ehrlich subcutaneous solid tumor growth by 60%. The results indicated therapeutic potential of immunogenic tumor-associated ganglioside in solid tumor model.

• Authors: S Saha, S Mondal
• Journal: Journal of experimental & clinical cancer research : CR (J Exp Clin Cancer Res) Vol. 20 Issue 1 Pg. 75-84 (Mar 2001) ISSN: 0392-9078 [Print] England
• PMID: 11370834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Anti-Idiotypic
• Gangliosides
• Immunoglobulin G
• Immunoglobulin M
• trisialoganglioside GT1

Topics

• Animals
• Antibodies, Anti-Idiotypic (therapeutic use)
• Antibody Formation
• Carcinoma, Ehrlich Tumor (blood, immunology, pathology, therapy)
• Gangliosides (blood, immunology)
• Immunoglobulin G (therapeutic use)
• Immunoglobulin M (therapeutic use)
• Immunotherapy (methods)
• Male
• Mice
• Rabbits