[Pilot study of pattern-electroretinographic changes in the DBA/2NNia mouse. Animal model of congenital angle-closure glaucoma].

Abstract

PURPOSE:

The aim of this study was to document the time-course of retinal dysfunction by pattern-electroretinography (PERG) in eyes of the DBA/2NNia substrain of mouse that develop an inherited angle-closure glaucoma.

METHODS:

Twelve DBA/2NNia mice and 12 control C57BL/6 J mice were studied by PERG recordings from 2 to 10 months of age. PERGs were recorded using different spatial and temporal frequencies.

RESULTS:

PERGs recorded with a temporal frequency of 7.5 Hz and a spatial frequency of 0.4 cycles/degree performed best to discriminate between DBA/2NNia mice and C57BL/6 J mice. When compared with normal C57BL/6 J mice, significant amplitude reductions of the PERG (Student's t-test; p < 0.01) were found in DBA/2NNia mice by 5 months of age and continued to decline as the animals aged. At beginning of follow-up, the mean PERG amplitude in DBA/2NNia mice was 2.3 +/- 0.5 microV. At 5 months of age, the mean PERG amplitude was reduced by 0.9 +/- 0.45 microV (paired t-test; p < 0.0001).

CONCLUSION:

Previously, a significant loss of retinal ganglion cells was found in the DBA/2NNia mouse substrain at 6-7 months of age. In the present study, we found decreases in PERG amplitudes, occurring from the age of 5 months onward. Similarities with the findings in human glaucoma indicate the relevance of this animal model for further glaucoma research.

• Authors: A U Bayer, S E Brodie, T Mittag
• Journal: Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft (Ophthalmologe) Vol. 98 Issue 3 Pg. 248-52 (Mar 2001) ISSN: 0941-293X [Print] Germany
• Vernacular Title: Pilotstudie zu musterelektroretinographischen Veränderungen bei der DBA/2NNia-Maus. Tiermodell eines angeborenen Engwinkelglaukoms.
• PMID: 11320811 (Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Topics

• Age Factors
• Animals
• Disease Models, Animal
• Electroretinography (methods)
• Follow-Up Studies
• Glaucoma, Angle-Closure (congenital, physiopathology)
• Mice
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Pilot Projects
• Research