PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors.

Abstract	In papillary renal cell carcinomas the TFE3 transcription factor becomes fused to the PSF and NonO pre-mRNA splicing factors and most commonly to a protein of unknown function designated PRCC. In this study we have examined the ability of the resulting PRCC-TFE3 and NonO-TFE3 fusions to activate transcription from the plasminogen activator inhibitor-1 (PAI-1) promoter. The results show that only fusion to PRCC enhanced transcriptional activation, indicating that the ability to enhance the level of transcription from endogenous TFE3 promoters is not a consistent feature of TFE3 fusions. In investigations of the normal function of PRCC we observed that PRCC expressed as a green fluorescent fusion protein colocalizes within the nucleus with Sm pre-mRNA splicing factors. It was also found that endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA splicing factors including SC35, PRL1 and CDC5. Association with the cellular splicing machinery is therefore, a common feature of the proteins that become fused to TFE3 in papillary renal cell carcinomas.
Authors	Y M Skalsky, P M Ajuh, C Parker, A I Lamond, G Goodwin, C S Cooper
Journal	Oncogene (Oncogene) Vol. 20 Issue 2 Pg. 178-87 (Jan 11 2001) ISSN: 0950-9232 [Print] England
PMID	11313942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Amanitins Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Cell Cycle Proteins DNA-Binding Proteins Enzyme Inhibitors Luminescent Proteins Neoplasm Proteins Nuclear Proteins PRCC protein, human Proteins RNA Precursors RNA, Messenger Recombinant Fusion Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae Proteins TFE3 protein, human Transcription Factors SRSF2 protein, human Green Fluorescent Proteins Serine-Arginine Splicing Factors Protein Kinases Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae
Topics	Adult Amanitins (pharmacology) Animals Artificial Gene Fusion Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Carcinoma, Papillary (drug therapy, genetics, metabolism) Carcinoma, Renal Cell (drug therapy, genetics, metabolism) Cell Cycle Proteins DNA-Binding Proteins (genetics, metabolism) Enzyme Inhibitors (pharmacology) Female Green Fluorescent Proteins Humans Kidney Neoplasms (drug therapy, genetics, metabolism) Luminescent Proteins (genetics, metabolism) Male Neoplasm Proteins Nuclear Proteins (metabolism) Protein Kinases (metabolism) Protein Serine-Threonine Kinases Proteins (genetics, metabolism) RNA Precursors (genetics) RNA Splicing RNA, Messenger (genetics) Recombinant Fusion Proteins (genetics, metabolism) Ribonucleoproteins Ribonucleoproteins, Small Nuclear (metabolism) Saccharomyces cerevisiae Proteins Serine-Arginine Splicing Factors Transcription Factors (genetics, metabolism) Transcription, Genetic Tumor Cells, Cultured

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