HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Vaccination of Balb/c mice against experimental visceral leishmaniasis with the GP36 glycoprotein antigen of Leishmania donovani.

Abstract
Leishmania donovani GP36 glycoprotein is the main antigen of the FML Fucose Mannose Ligand (FML) complex specifically recognized by sera of kala-azar human patients. The GP36 was isolated by chemical elution + sonication and used for Balb/c mouse vaccination in combination with saponin, by the s.c. route, inducing a strong and specific protective effect against experimental visceral leishmaniasis shown by the increase of: specific IgG antibodies (82.6%), mainly IgG2a, the delayed type of hypersensitivity to promastigote lysate (37.8%, P < 0.001), the in vitro cellular proliferative response to GP36 of ganglia lymphocytes (53.5%, P < 0.005) and the decrease of liver parasite burden (68.1%, P < 0.025). Saponin treated controls reacted significantly differently from GP36 vaccinated animals at all the assayed variables (P < 0.05). GP36 induced significant protection against murine visceral leishmaniasis at concentrations commonly used for vaccination with recombinant antigens.
AuthorsE Paraguai de Souza, R R Bernardo, M Palatnik, C B Palatnik de Sousa
JournalVaccine (Vaccine) Vol. 19 Issue 23-24 Pg. 3104-15 (Apr 30 2001) ISSN: 0264-410X [Print] Netherlands
PMID11312005 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Protozoan Proteins
  • Protozoan Vaccines
Topics
  • Animals
  • Antibodies, Protozoan (biosynthesis)
  • Antigens, Protozoan (administration & dosage, isolation & purification)
  • Female
  • Immunity, Cellular
  • Leishmania donovani (immunology)
  • Leishmaniasis, Visceral (immunology, prevention & control)
  • Mice
  • Mice, Inbred BALB C
  • Protozoan Proteins (administration & dosage, immunology, isolation & purification)
  • Protozoan Vaccines (administration & dosage)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: