Regulation of matrix metalloproteinases and their inhibitors in uterine endometrial cells of patients with and without endometriosis.

Abstract

OBJECTIVE:

To determine whether alterations in the secretion and regulation of matrix metalloproteinases (MMPs) and their inhibitors are present in uterine endometrial cells from endometriosis patients.

STUDY DESIGN:

In an in vitro study, uterine endometrial cells from 19 regularly cycling women with and 32 without endometriosis were treated with diethyl stilbestrol, promegestone (R5020), interleukin-1 (IL-1) and tumor necrosis factor a (TNF-alpha). Culture supernatants were assayed for MMPs 1, 2, 3, and 9, and for tissue inhibitors of MMP (TIMP-1 and TIMP-2) by ELISA.

RESULTS:

MMP-3 was secreted in high concentrations, moderate concentrations were seen for MMP-1 and MMP-2, and very low concentrations for MMP-9. Substantially more TIMP-1 than TIMP-2 was secreted. MMP-1 and MMP-3 were uniformly attenuated by R5020, while MMP-2 was not influenced by hormone treatment. MMP-3 was upregulated by TNF-alpha in all samples while IL-1 only increased secretion in cells from endometriosis patients.

CONCLUSION:

The upregulation of MMP-3 by IL-1 may contribute to an increased invasiveness of uterine endometrial fragments in endometriosis patients.

• Authors: M Sillem, S Prifti, A Koch, M Neher, J Jauckus, B Runnebaum
• Journal: European journal of obstetrics, gynecology, and reproductive biology (Eur J Obstet Gynecol Reprod Biol) Vol. 95 Issue 2 Pg. 167-74 (Apr 2001) ISSN: 0301-2115 [Print] Ireland
• PMID: 11301163 (Publication Type: Journal Article)

Chemical References

• Interleukin-1
• Tissue Inhibitor of Metalloproteinase-1
• Tissue Inhibitor of Metalloproteinases
• Tumor Necrosis Factor-alpha
• Tissue Inhibitor of Metalloproteinase-2
• Diethylstilbestrol
• Promegestone
• Matrix Metalloproteinases
• Matrix Metalloproteinase 3
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• Matrix Metalloproteinase 1

Topics

• Adult
• Cells, Cultured
• Diethylstilbestrol (pharmacology)
• Endometriosis (enzymology)
• Endometrium (enzymology)
• Female
• Humans
• Interleukin-1 (pharmacology)
• Matrix Metalloproteinase 1 (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 3 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Matrix Metalloproteinases (metabolism)
• Promegestone (pharmacology)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)
• Tissue Inhibitor of Metalloproteinase-2 (metabolism)
• Tissue Inhibitor of Metalloproteinases (metabolism)
• Tumor Necrosis Factor-alpha (pharmacology)