In vitro studies have suggested that
losartan interacts with the
thromboxane (TxA2)/
prostaglandin H2 (
PGH2) receptor in human platelets, reducing TxA2-dependent platelet activation. The aim of this study was to evaluate the effect of different
angiotensin II type 1 receptor antagonists in
stroke-prone spontaneously hypertensive rats (SHRSP). The level of platelet activation was assessed by determining
P-selectin expression in platelets by flow cytometry. The ex vivo adhesion of platelets was also analyzed. The number of platelets that expressed
P-selectin in SPSHR was significantly increased (%
P-selectin expression: WKY 4 +/- 0, 4%; SHRSP 15.5 +/- 0, 8% [n = 8], p < 0.05). In SHRSP receiving
losartan (20 mg/kg
body weight per day) the percentage of platelets expressing
P-selectin fell to levels close to that observed in WKY. The number of platelets from SHRSP treated with
valsartan and
candesartan (20 mg/kg
body weight per day for 14 days) that expressed
P-selectin was not significantly different from those from untreated SPRHR. Only
losartan treatment reduced ex vivo platelet adhesion to a synthetic surface. The antiplatelet effect of
losartan does not appear to be related to the level of blood pressure reduction. In ex vivo experiments,
losartan significantly reduced the binding of the radiolabeled TxA2 agonist
U46619 to platelets obtained from SHRSP in a dose-dependent manner. Treatment with
losartan reduced the number of activated platelets in SHRSP independently of its blood pressure effects. TxA2-receptor blockade is proposed as a mechanism by which
losartan can prevent platelet activation.