HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Visualization of immunotoxin-mediated tumor cell death in vivo.

Abstract
We present a novel methodology to visualize tumor cells directly in a whole mouse. This technique combines immunohistochemistry with whole mouse sectioning. It lets one see the exact distribution of tumor cells throughout an animal and how effectively these cells are eliminated by cancer therapeutics. We used this technique to assess the efficacy of a T cell-specific immunotoxin in a severe combined immunodeficient mouse model of human T-cell leukemia. Severe combined immunodeficient mice were injected with one of two human T-cell acute lymphoblastic leukemia cell lines (Molt 3 and Molt 13) and were either left untreated or were treated with DA7, an immunotoxin specific for the T cell-associated antigen CD7. Mice were sacrificed after tumor cell injection and immunotoxin therapy, whole mouse cross-sections were prepared, and tumor cells in the sections were visualized by immunohistochemistry. No tumor cells were detected in DA7-treated mice injected with Molt 3, consistent with the long-term survival of this group and the sensitivity of Molt 3 to DA7 in vitro. In contrast, DA7 treatment did not visibly eliminate tumor cells in mice challenged with Molt 13, nor did it result in their long-term survival. Furthermore, tumor cells were detected in areas that may have otherwise been overlooked, and their distribution differed from that of mice injected with Molt 13 alone. These analyses indicate that whole mouse sectioning will be a valuable tool for assessing residual disease in the preclinical evaluation of cancer therapeutics.
AuthorsH A Erickson, R L Reinhardt, J B Hermanson, A Panoskaltsis-Mortari, C A Pennell
JournalClinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 7 Issue 3 Suppl Pg. 890s-894s (Mar 2001) ISSN: 1078-0432 [Print] United States
PMID11300488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, CD7
  • Immunotoxins
Topics
  • Animals
  • Antigens, CD7 (metabolism)
  • Cell Death
  • Cell Line
  • Dose-Response Relationship, Drug
  • Humans
  • Immunohistochemistry
  • Immunotoxins (pharmacology)
  • Leukemia, T-Cell (pathology)
  • Mice
  • Mice, SCID
  • Neoplasms (pathology, therapy)
  • Time Factors
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: