A prime-boost vaccination strategy using naked DNA followed by recombinant porcine adenovirus protects pigs from classical swine fever.

Abstract

Weaned pigs (6-week-old) and 7-day-old pre-weaned piglets were vaccinated with naked plasmid DNA expressing the gp55/E2 gene from classical swine fever virus (CSFV). Both groups of pigs were then given a booster dose of recombinant porcine adenovirus expressing the gp55 gene (rPAV-gp55). Following challenge with CSFV, 100% of weaned pigs and 75% pre-weaned piglets were protected from disease. Weaned pigs given a single dose of rPAV-gp55 were also protected, but showed a slight increase in temperature immediately post-challenge. However, weaned animals given a DNA prime before rPAV-gp55 showed no fluctuation in body temperature following challenge and no pathology in spleen or lymph nodes upon post-mortem. In addition, no CSFV could be re-isolated from the rPAV vaccinated group and from only one pig in the prime-boost group following challenge, suggesting that both vaccination regimes have the potential to reduce or prevent virus shedding following experimental challenge.

• Authors: J M Hammond, E S Jansen, C J Morrissy, W V Goff, G C Meehan, M M Williamson, C Lenghaus, K W Sproat, M E Andrew, B E Coupar, M A Johnson
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 80 Issue 2 Pg. 101-19 (May 21 2001) ISSN: 0378-1135 [Print] Netherlands
• PMID: 11295331 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Recombinant
• Vaccines, DNA
• Viral Envelope Proteins
• glycoprotein E2, classical swine fever virus

Topics

• Adenoviridae
• Animals
• Body Temperature
• Classical Swine Fever (prevention & control)
• DNA, Recombinant (administration & dosage)
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Swine
• Vaccination (veterinary)
• Vaccines, DNA
• Viral Envelope Proteins (genetics, immunology)
• Weaning