Pretreatment with glycine reduces the severity of warm intestinal ischemic-reperfusion injury in the rat.

Abstract

Free jejunal flaps may experience adverse effects immediately after revascularization because of ischemic-reperfusion injury. In this study the authors evaluated the ability of glycine to protect the small intestine against the effects of a warm ischemic-reperfusion injury. Male Wistar rats (N = 30) were randomized to either a baseline group (no intervention), a control group (local arterial infusion with normal saline), or a glycine group (local arterial infusion with 20% glycine). Pretreatment with 20% glycine increased significantly (p < 0.05) mucosal protein and deoxyribonucleic acid content, reduced intestinal myeloperoxidase activity, and maintained mucosal glutaminase activity. These results indicate that some of the indicators of ischemic-reperfusion injury are improved by pretreatment with a 20% glycine solution.

• Authors: M A Lee, R D McCauley, S E Kong, J C Hall
• Journal: Annals of plastic surgery (Ann Plast Surg) Vol. 46 Issue 3 Pg. 320-6 (Mar 2001) ISSN: 0148-7043 [Print] United States
• PMID: 11293527 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycine

Topics

• Animals
• Chi-Square Distribution
• Glycine (administration & dosage, pharmacology)
• Infusions, Intra-Arterial
• Ischemia (pathology)
• Jejunum (blood supply, drug effects)
• Male
• Rats
• Rats, Wistar
• Reperfusion Injury (pathology, prevention & control)
• Statistics, Nonparametric
• Surgical Flaps (blood supply)