Jaagsiekte sheep retrovirus proviral clone JSRV(JS7), derived from the JS7 lung tumor cell line, induces ovine pulmonary carcinoma and is integrated into the surfactant protein A gene.

Ovine pulmonary carcinoma (OPC) is a contagious neoplasm of alveolar epithelial type II (ATII) or Clara cells caused by a type D/B chimeric retrovirus, jaagsiekte sheep retrovirus (JSRV). Here we report the isolation, sequencing, pathogenicity, and integration site of a JSRV provirus isolated from a sheep lung tumor cell line (JS7). The sequence of the virus was 93 to 99% identical to other JSRV isolates and contained all of the expected open reading frames. To produce virions and test its infectivity, the JS7 provirus (JSRV(JS7)) was cloned into a plasmid containing a cytomegalovirus promoter and transfected into 293T cells. After intratracheal inoculation with virions from concentrated supernatant fluid, JSRV-associated OPC lesions were found in one of four lambs, confirming that JSRV(JS7) is pathogenic. In JS7-cell DNA, the viral genome was inserted in the protein-coding region for the surfactant protein A (SP-A) gene, which is highly expressed in ATII cells, in an orientation opposite to the direction of transcription of the SP-A gene. No significant transcription was detected from either the viral or the SP-A gene promoter in the JS7 cell line at passage level 170. The oncogenic significance of the JSRV proviral insertion involving the SP-A locus in the JS7 tumor cell line is unknown.
AuthorsJ C DeMartini, J V Bishop, T E Allen, F A Jassim, J M Sharp, M de las Heras, D R Voelker, J O Carlson
JournalJournal of virology (J Virol) Vol. 75 Issue 9 Pg. 4239-46 (May 2001) ISSN: 0022-538X [Print] United States
PMID11287573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Viral
  • Proteolipids
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line, Transformed
  • DNA, Viral
  • Humans
  • Jaagsiekte sheep retrovirus (genetics, isolation & purification, pathogenicity)
  • Lung Neoplasms
  • Molecular Sequence Data
  • Proteolipids (genetics)
  • Proviruses (genetics, isolation & purification)
  • Pulmonary Adenomatosis, Ovine (virology)
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants (genetics)
  • Sheep
  • Tumor Cells, Cultured
  • Virus Integration (genetics)

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