Granulocyte colony-stimulating factor (G-CSF) receptor gene expression of ovarian carcinoma does not correlate with G-CSF caused cell proliferation.

Abstract

BACKGROUND:

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is a growth factor commonly used to avoid leukopenia after chemotherapy. Endogenous G-CSF is produced by macrophages and granulocytes that infiltrate tumors. It has been reported that rhG-CSF stimulates the proliferation of several cell lines as well as bladder carcinoma cells. Conversely, in some hematopoietic cell lines such as U-937, WEHI-3B, and K-562 no effect or in some cases a differentiation pattern was found. Moreover, the role of rhG-CSF on the proliferation of solid tumors is not well understood.

METHODS:

In this study, 10 ovarian carcinoma biopsies were characterized for the presence of G-CSF and G-CSF receptor by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis. Proliferation was analyzed by ATP viability assays.

RESULTS:

Performing RT-PCR, these biopsies and four ovarian carcinoma cell lines were analyzed for endogenous G-CSF production, which was found in some biopsies and in all cell lines. Despite the presence of the G-CSF receptor in all biopsies and cell lines, no proliferation was found after rhG-CSF incubation of the cell lines or the tumor samples for 3 and for 6 days, respectively.

CONCLUSIONS:

Summarizing the authors' in vitro studies, rhG-CSF does not affect the proliferation of ovarian carcinoma cells in vitro.

• Authors: T Brandstetter, E Ninci, H Bettendorf, G Perewusnyk, J Stolte, D Herchenbach, D Sellin, E Wagner, O R Köchli, T Bauknecht
• Journal: Cancer (Cancer) Vol. 91 Issue 7 Pg. 1372-83 (Apr 01 2001) ISSN: 0008-543X [Print] United States
• PMID: 11283939 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 American Cancer Society.

Chemical References

• Colony-Stimulating Factors
• Receptors, Granulocyte Colony-Stimulating Factor
• Recombinant Proteins
• Granulocyte Colony-Stimulating Factor
• Adenosine Triphosphate

Topics

• Adenosine Triphosphate (analysis)
• Biopsy
• Blotting, Southern
• Carcinoma (chemistry, genetics, pathology)
• Cell Division
• Colony-Stimulating Factors (pharmacology)
• Female
• Gene Expression
• Granulocyte Colony-Stimulating Factor (analysis, physiology)
• Humans
• Immunohistochemistry
• Ovarian Neoplasms (chemistry, genetics, pathology)
• Receptors, Granulocyte Colony-Stimulating Factor (analysis, genetics)
• Recombinant Proteins
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured