Effect of tetrandrine on morphine dependence in isolated guinea pig ileum.

Abstract

AIM:

To evaluate the effects of tetrandrine (Tet) and nimodipine (Nim) on the morphine (Mor) withdrawal response in the isolated guinea pig ileum.

METHODS:

The withdrawal contracture was elicited by addition of naloxone (Nal) (1 mumol.L-1) to the isolated naive ileum incubated with Mor (3 mumol.L-1) at 37.5 degrees C for 4 h or to the ileum obtained from Mor-dependent guinea pig.

RESULTS:

When Nim (0.01, 0.05, and 0.1 mumol.L-1) or Tet (1, 10, and 50 mumol.L-1) was added 1 min before Nal in the naive ilea bathed in Krebs solution containing Mor, or when the ilea from Mor-dependent guinea pigs were incubated with Nim (0.01, 0.05, and 0.1 mumol.L-1) or Tet (1, 10, and 50 mumol.L-1) for 15 min, or when Nim (5 and 10 mg.kg-1, i.p.) or Tet (15 and 30 mg.kg-1, i.p.) was administered in vivo to Mor-dependent guinea pigs, the Nal-precipitated withdrawal contracture was significantly decreased in a dose-dependent manner.

CONCLUSION:

Tet and Nim, Ca2+ channel blockers, could inhibit the Nal-precipitated Mor withdrawal response in the isolated guinea pig ileum.

• Authors: Z H Liu, J L Li, N Li, C L Tian, K G Zhang
• Journal: Zhongguo yao li xue bao = Acta pharmacologica Sinica (Zhongguo Yao Li Xue Bao) Vol. 20 Issue 11 Pg. 1000-4 (Nov 1999) ISSN: 0253-9756 [Print] China
• PMID: 11270964 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Benzylisoquinolines
• Calcium Channel Blockers
• tetrandrine
• Nimodipine

Topics

• Alkaloids (pharmacology)
• Animals
• Benzylisoquinolines
• Calcium Channel Blockers (pharmacology)
• Guinea Pigs
• Ileum (drug effects)
• In Vitro Techniques
• Male
• Morphine Dependence (physiopathology)
• Muscle Contraction (drug effects)
• Muscle, Smooth (drug effects)
• Nimodipine (pharmacology)
• Substance Withdrawal Syndrome (physiopathology)