Lack of HLA-class I antigens in human neuroblastoma cells: analysis of its relationship to TAP and tapasin expression.

Abstract

We studied the constitutive and the interferon (IFN)-gamma-induced expression of HLA class I antigen heavy chain, beta2-microglobulin (beta2m), TAP-1, TAP-2 and tapasin in a panel of eleven neuroblastoma cell lines. Surface expression of HLA class I antigens was low in eight out of eight neuroblastoma cell lines bearing MYC-N amplification and/or 1p deletion, while two out of three neuroblastoma cell lines lacking these genetic alterations showed normal expression. IFN-gamma treatment restored HLA class I antigen surface expression in all neuroblastoma cell lines. Eight out of 11 neuroblastoma cell lines did not express TAP-1 mRNA and three of them also lacked TAP-2 mRNA. beta2 m mRNA was barely detectable or absent in five neuroblastoma cell lines, while tapasin mRNA was always expressed. IFN-gamma upregulated the expression of HLA class I heavy chain, beta2 m, TAP-1, TAP-2 and tapasin, as detected at mRNA or protein level. Post-transcriptional events were involved in altered TAP-1 and beta2 m expression in one peculiar neuroblastoma cell line. These data indicate that multiple mechanisms play a role in the HLA class I antigen-deficient phenotype of human neuroblastoma.

• Authors: M V Corrias, M Occhino, M Croce, A De Ambrosis, M P Pistillo, P Bocca, V Pistoia, S Ferrini
• Journal: Tissue antigens (Tissue Antigens) Vol. 57 Issue 2 Pg. 110-7 (Feb 2001) ISSN: 0001-2815 [Print] England
• PMID: 11260505 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 2
• ATP Binding Cassette Transporter, Subfamily B, Member 3
• ATP-Binding Cassette Transporters
• Antigens, Surface
• Antineoplastic Agents
• Antiporters
• Extracellular Matrix Proteins
• Histocompatibility Antigens Class I
• Immunoglobulin Heavy Chains
• Immunoglobulins
• Membrane Transport Proteins
• Nerve Tissue Proteins
• RNA, Messenger
• TAP1 protein, human
• beta 2-Microglobulin
• tapasin
• TAP2 protein, human
• Interferon-gamma

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 2
• ATP Binding Cassette Transporter, Subfamily B, Member 3
• ATP-Binding Cassette Transporters (analysis, genetics, immunology)
• Antigens, Surface (genetics, immunology)
• Antineoplastic Agents (pharmacology)
• Antiporters (analysis, genetics, immunology)
• Blotting, Western
• Brain Neoplasms (immunology)
• Extracellular Matrix Proteins (analysis, genetics, immunology)
• Gene Deletion
• Gene Expression (drug effects, immunology)
• Genes, myc
• Histocompatibility Antigens Class I (analysis, genetics, immunology)
• Humans
• Immunoglobulin Heavy Chains (analysis, genetics, immunology)
• Immunoglobulins (analysis, genetics, immunology)
• Interferon-gamma (pharmacology)
• Membrane Transport Proteins
• Nerve Tissue Proteins (analysis, genetics, immunology)
• Neuroblastoma (immunology)
• RNA, Messenger (analysis)
• Tumor Cells, Cultured
• beta 2-Microglobulin (analysis, genetics, immunology)