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Suppression of gingival inflammation induced by Porphyromonas gingivalis in rats by leupeptin.

Abstract
In this study, we developed a procedure to produce gingivitis in rats by inoculation of Porphyromonas gingivalis and studied the contribution of the bacterial cysteine proteinases, Arg-gingipain (Rgp) and Lys-gingipain (Kgp), to the pathology in the gingiva. To adhere the bacterium to periodontal tissues, a cotton thread was inserted between the first and second molar of right maxillary sites of rats. Rats in group A were administered with vehicle alone after bacterial (strain W83) inoculation. In group B, the bacteria were inoculated in combination with leupeptin, a potent inhibitor of Rgp and Kgp, and then leupeptin alone was administered the week after. Rats in group C were administered leupeptin for 6 weeks after bacteria inoculation. All left maxillary gingiva in three groups showed no inflammatory changes. Right maxillary gingiva of group A showed most of the clinical landmarks of gingivitis. Leupeptin exhibited only a little inhibitory effect on this gingivitis in group B, whereas it had a strong inhibitory effect on the inflammation in group C. These results suggest that P. gingivalis-induced gingivitis is attributable to Rgp and Kgp and that leupeptin is more effective in the late phase than the early stage of gingivitis.
AuthorsS Kitano, K Irimura, T Sasaki, N Abe, A Baba, Y Miyake, N Katunuma, K Yamamoto
JournalJapanese journal of pharmacology (Jpn J Pharmacol) Vol. 85 Issue 1 Pg. 84-91 (Jan 2001) ISSN: 0021-5198 [Print] Japan
PMID11243579 (Publication Type: Journal Article)
Chemical References
  • Adhesins, Bacterial
  • Cysteine Proteinase Inhibitors
  • Gingipain Cysteine Endopeptidases
  • Hemagglutinins
  • Leupeptins
  • Cysteine Endopeptidases
  • leupeptin
Topics
  • Adhesins, Bacterial (drug effects, metabolism)
  • Animals
  • Bacteroidaceae Infections (drug therapy, microbiology)
  • Cysteine Endopeptidases (drug effects, metabolism)
  • Cysteine Proteinase Inhibitors (pharmacology, therapeutic use)
  • Disease Models, Animal
  • Disease Progression
  • Gingipain Cysteine Endopeptidases
  • Gingivitis (drug therapy, microbiology, pathology)
  • Hemagglutinins (drug effects, metabolism)
  • Leupeptins (pharmacology, therapeutic use)
  • Male
  • Maxilla (pathology)
  • Porphyromonas gingivalis (enzymology, pathogenicity)
  • Rats
  • Rats, Wistar
  • Virulence

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