Abstract | OBJECTIVE: METHODS:
Cyclin D1 overexpression in 23 dysplastic nevi was detected by an immunohistochemical technique. The extent of atypia of the nevi was graded as mild, moderate, or severe, using previously established criteria. RESULTS:
Cyclin D1 overexpression in dysplastic nevi maintained a zonal pattern, similar to Spitz nevi. Cyclin D1 overexpression was greatest in the region of the epidermal-dermal junction and was significantly less prominent in the papillary and reticular dermis, suggesting that cyclin D1 expression is under cell control and correlates with maturation of nevus cells. Cyclin D1 overexpression also correlated with cytologic atypia, as dysplastic nevi with moderate or severe cytologic atypia contained a greater percentage of cyclin D1-positive cells than did nevi with mild atypia. Six dysplastic nevi with many cyclin D1--positive cells were assessed by fluorescence in situ hybridization studies using cyclin D1--specific and chromosome 11 centromeric probes. In all cases, there was no evidence of 11q13 translocation, amplification, or trisomy of chromosome 11. CONCLUSIONS:
|
Authors | C Ewanowich, R K Brynes, L Medeiros, A McCourty, R Lai |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 125
Issue 2
Pg. 208-10
(Feb 2001)
ISSN: 0003-9985 [Print] United States |
PMID | 11175636
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Chromosomes, Human, Pair 11
- Cyclin D1
(analysis, genetics)
- Dysplastic Nevus Syndrome
(genetics, metabolism, pathology)
- Humans
- Immunohistochemistry
- In Situ Hybridization, Fluorescence
- Melanocytes
(chemistry)
- Tissue Distribution
|