Abstract |
The mechanism of weight loss induced by the growth of malignant tumors is still unknown. We investigated it by focusing on apoptosis of skeletal muscle. VX2-tumor was implanted into rabbits and the apoptotic index (AI) of skeletal muscle was measured by in situ end-labeling assay. Plasma of the tumor-bearing rabbits was perfused repeatedly through non-coated charcoal resin. The AI reached 54.6% early after tumor implantation, when weight loss amounted to an 18% decrease in lean body mass (LBM) without change in muscle DNA synthesis or urinary 3-methylhistidine / creatinine ratio (3-MH / Cr). When the decrease of LBM reached 30%, DNA synthesis was decreased by 48% and 3-MH / Cr was increased by 104%, whereas AI was only 4.7%. The plasma perfusion did not prevent apoptosis in muscle, but improved LBM, DNA synthesis, and 3-MH / Cr. There may be two mechanisms of muscle depletion during the tumor growth: apoptosis in the early stage and metabolic abnormalities in muscle in the late stage.
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Authors | O Ishiko, T Sumi, K Hirai, K I Honda, S Nakata, H Yoshida, S Ogita |
Journal | Japanese journal of cancer research : Gann
(Jpn J Cancer Res)
Vol. 92
Issue 1
Pg. 30-5
(Jan 2001)
ISSN: 0910-5050 [Print] Japan |
PMID | 11173541
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Methylhistidines
- DNA
- Creatinine
- 3-methylhistidine
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Topics |
- Animals
- Apoptosis
- Body Mass Index
- Creatinine
(urine)
- DNA
(biosynthesis)
- DNA Fragmentation
- In Situ Nick-End Labeling
- Male
- Methylhistidines
(urine)
- Muscle, Skeletal
(cytology, metabolism, physiopathology)
- Neoplasms
(metabolism, physiopathology, urine)
- Rabbits
- Weight Loss
(genetics, physiology)
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