Transfer of myelin-specific cells deviated in vitro towards IL-4 production ameliorates ongoing experimental allergic neuritis.

Abstract

A causal role of IL-4 (Th2) production for recovery in experimental allergic neuritis (EAN) was indicated by experiments where Th1-like autoreactive cell populations, taken from the induction phase of the disease, were deviated to extensive secretion of IL-4 in a selective fashion, by ex vivo stimulation with autoantigen in the presence of IL-4. The deviated cells were adoptively transferred to EAN rats at a time just prior to the onset of clinical signs. This treatment ameliorated EAN compared with sham treatment. This therapeutic approach, with generation of autoreactive IL-4-secreting cells ex vivo followed by subsequent adoptive transfer, may become a new selective treatment of organ-specific autoimmune diseases since, in contrast to previous attempts, it is done in a physiological and technically easy way.

• Authors: C Ekerfelt, C Dahle, R Weissert, M Kvarnström, T Olsson, J Ernerudh
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 123 Issue 1 Pg. 112-8 (Jan 2001) ISSN: 0009-9104 [Print] England
• PMID: 11168007 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Epitopes, T-Lymphocyte
• Myelin P2 Protein
• Peptide Fragments
• Recombinant Proteins
• myelin P2 peptide SP26
• Interleukin-4

Topics

• Adoptive Transfer (methods)
• Animals
• Cytokines (metabolism)
• Epitopes, T-Lymphocyte (immunology, toxicity)
• Interleukin-4 (biosynthesis, genetics, pharmacology)
• Lymph Nodes (cytology, immunology, metabolism)
• Lymphocyte Transfusion
• Male
• Myelin P2 Protein (immunology, toxicity)
• Neuritis, Autoimmune, Experimental (immunology, metabolism, prevention & control)
• Peptide Fragments (immunology, toxicity)
• Rats
• Rats, Inbred Lew
• Recombinant Proteins (pharmacology)
• T-Lymphocyte Subsets (immunology, metabolism, transplantation)
• Th2 Cells (immunology, metabolism, transplantation)