Abstract | AIMS/HYPOTHESIS: METHODS: GLUT2 expressing RINm5F cells were generated through stable transfection of the rat glucose transporter GLUT2 cDNA under the control of the cytomegalovirus promoter in the pcDNA3 vector. Viability of the cells was determined using a microtitre plate-based 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyl tetrazolium bromide (MTT) assay. RESULTS: CONCLUSION/INTERPRETATION:
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Authors | M Elsner, B Guldbakke, M Tiedge, R Munday, S Lenzen |
Journal | Diabetologia
(Diabetologia)
Vol. 43
Issue 12
Pg. 1528-33
(Dec 2000)
ISSN: 0012-186X [Print] Germany |
PMID | 11151762
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Alkylating Agents
- Glucose Transporter Type 2
- Monosaccharide Transport Proteins
- Recombinant Proteins
- 3-O-Methylglucose
- Streptozocin
- Methylnitrosourea
- Ethyl Methanesulfonate
- Methyl Methanesulfonate
- Ethylnitrosourea
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Topics |
- 3-O-Methylglucose
(pharmacokinetics)
- Alkylating Agents
(pharmacokinetics, toxicity)
- Alkylation
- Animals
- Biological Transport
- Biotransformation
- Cell Line
- Cell Survival
(drug effects)
- Ethyl Methanesulfonate
(toxicity)
- Ethylnitrosourea
(toxicity)
- Glucose Transporter Type 2
- Islets of Langerhans
(drug effects, pathology)
- Kinetics
- Methyl Methanesulfonate
(toxicity)
- Methylnitrosourea
(toxicity)
- Monosaccharide Transport Proteins
(genetics, metabolism)
- Rats
- Recombinant Proteins
(metabolism)
- Streptozocin
(pharmacokinetics, toxicity)
- Transfection
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