A tetravalent single-chain antibody-streptavidin fusion protein for pretargeted lymphoma therapy.

Abstract

Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of Escherichia coli. Expression levels were affected by the order of the variable regions and the length and composition of the single-chain Fv linker. The best expressor was obtained with the variable regions in the heavy chain-light chain configuration separated by a 25-mer Gly4Ser linker. This construct produced 250-300 mg of soluble, tetrameric fusion protein per liter of fermentor culture. The fusion protein (Mr 173,600) was purified from crude lysates by iminobiotin affinity chromatography with an overall yield of about 50% and was analyzed for functionality both in vitro and in vivo. Immunoreactivity of the scFvSA fusion protein and its nanomolar affinity to CD20-positive Ramos cells were comparable with the B9E9 monoclonal antibody. The fusion protein had a biotin dissociation rate identical to recombinant streptavidin and bound an average of 3.6 biotins/molecule of a possible 4 biotins/molecule. Labeled fusion protein cleared from the blood of BALB/c mice with a P half-life of about 16 h. In nude mice bearing Ramos xenografts, the fusion protein demonstrated sufficient tumor localization of functional streptavidin to enable efficient, tumor-specific targeting of a subsequently administered radionuclide-chelate/biotin molecule. These results suggest that large quantities of functional scFvSA can be produced for clinical testing as a therapy for non-Hodgkin's lymphoma.

• Authors: J Schultz, Y Lin, J Sanderson, Y Zuo, D Stone, R Mallett, S Wilbert, D Axworthy
• Journal: Cancer research (Cancer Res) Vol. 60 Issue 23 Pg. 6663-9 (Dec 01 2000) ISSN: 0008-5472 [Print] United States
• PMID: 11118050 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD20
• Immunoglobulin Fragments
• Iodine Radioisotopes
• Recombinant Fusion Proteins
• Streptavidin

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal (genetics, immunology, metabolism)
• Antigens, CD20 (immunology)
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli (genetics, metabolism)
• Female
• Humans
• Immunoglobulin Fragments (genetics, immunology, isolation & purification, metabolism)
• Iodine Radioisotopes (administration & dosage)
• Lymphoma, Non-Hodgkin (radiotherapy, therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Molecular Sequence Data
• Protein Engineering
• Radioimmunotherapy
• Recombinant Fusion Proteins (genetics, isolation & purification, metabolism)
• Sequence Homology, Amino Acid
• Streptavidin (genetics, isolation & purification, metabolism)
• Tissue Distribution
• Xenograft Model Antitumor Assays