Characterization of the T cell receptor repertoire in patients with common variable immunodeficiency: oligoclonal expansion of CD8(+) T cells.

Abstract

Common variable immunodeficiency is characterized by an impairment in antibody production of at least two immunoglobulin isotypes, and abnormalities of both B and T cells have also been described. In this study, a profound alteration of the T cell receptor repertoire was noted, especially in CD8(+) T cells, in CVID patients when compared to a control group. However, the higher number of oligoclonally expanded populations seen in the patient group was not dependent on age, whereas in the control group an age-related accumulation of these populations was noted. Expansion of CD8(+) CD28(-) T cells in these patients is strongly correlated with T cell receptor repertoire restriction. Furthermore, analysis of cytokine production showed a statistically significant increase in IFN-gamma secretion in the patient group. Lastly, CD94 and perforin were expressed at increased frequencies on CD8(+) T cells in the patient group when compared to the control group. We speculate that these findings support the concept that CD8(+) T cells may play an important regulatory role in CVID.

• Authors: D Serrano, K Becker, C Cunningham-Rundles, L Mayer
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 97 Issue 3 Pg. 248-58 (Dec 2000) ISSN: 1521-6616 [Print] United States
• PMID: 11112364 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• CD28 Antigens
• Cytokines
• Receptors, Antigen, T-Cell
• Interferon-gamma

Topics

• Adult
• Aged
• CD28 Antigens (analysis)
• CD8-Positive T-Lymphocytes (chemistry, metabolism)
• Clone Cells (cytology)
• Common Variable Immunodeficiency (metabolism, pathology)
• Cytokines (biosynthesis)
• Female
• Humans
• Interferon-gamma (metabolism)
• Male
• Middle Aged
• Receptors, Antigen, T-Cell (isolation & purification)