ERBB2 (HER-2/neu) amplification and/or overexpression are associated with poor prognosis in node-positive
breast carcinoma. Its prognostic value in node-negative cases and its predictive value for response to
chemotherapy remain controversial. This may be related to the use of molecular methods, which are sensitive to dilution of
tumor material by normal cells, or the use of nonstandardized immunohistochemistry (IHC) procedures, for the determination of the ERBB2 gene status. In addition, new therapeutic approaches that target the cells overexpressing ERBB2 are under development. These perspectives necessitate a reliable evaluation of the status of ERBB2 in individual
tumors before the application of specific therapeutic strategies. Fluorescent in situ hybridization (FISH) and IHC allow the evaluation of the ERBB2 status specifically in
tumor cells on archival material. We have analyzed a series of 100 invasive ductal
breast carcinomas without lymph node invasion both by IHC, using the CB11
monoclonal antibody and a sensitive Auidin
Biotin Complex (ABC) immunodetection system, and by FISH, using the Oncor Inform HER-21neu (ERBB2) gene amplification detection system as reference technique. Complete concordance between the results of FISH and IHC was seen in 98% of the cases. ERBB2 amplification (more than four signals per nucleus) was observed in 12 of the 100 cases, and all but one showed an overexpression of the
protein (membrane staining) by IHC. Conversely, ERBB2 expression was present in one case without gene amplification. In conclusion, ERBB2 overexpression detected by IHC is highly correlated to gene amplification detected by FISH. Thus, under standardized conditions, IHC is a reliable and economical test to assess the ERBB2 status in
tumors. The use of FISH could be limited to the verification of the status of
tumors displaying a weak membrane immunostaining.