Abstract |
Abscesses are a classic host response to infection by many pathogenic bacteria. The immunopathogenesis of this tissue response to infection has not been fully elucidated. Previous studies have suggested that T cells are involved in the pathologic process, but the role of these cells remains unclear. To delineate the mechanism by which T cells mediate abscess formation associated with intra-abdominal sepsis, the role of T-cell activation and the contribution of antigen-presenting cells via CD28-B7 costimulation were investigated. T cells activated in vitro by zwitterionic bacterial polysaccharides (Zps) known to induce abscess formation required CD28-B7 costimulation and, when adoptively transferred to the peritoneal cavity of naïve rats, promoted abscess formation. Blockade of T-cell activation via the CD28-B7 pathway in animals with CTLA4Ig prevented abscess formation following challenge with different bacterial pathogens, including Staphylococcus aureus, Bacteroides fragilis, and a combination of Enterococcus faecium and Bacteroides distasonis. In contrast, these animals had an increased abscess rate following in vivo T-cell activation via CD28 signaling. Abscess formation in vivo and T-cell activation in vitro required costimulation by B7-2 but not B7-1. These results demonstrate that abscess formation by pathogenic bacteria is under the control of a common effector mechanism that requires T-cell activation via the CD28-B7-2 pathway.
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Authors | A O Tzianabos, A Chandraker, W Kalka-Moll, F Stingele, V M Dong, R W Finberg, R Peach, M H Sayegh |
Journal | Infection and immunity
(Infect Immun)
Vol. 68
Issue 12
Pg. 6650-5
(Dec 2000)
ISSN: 0019-9567 [Print] United States |
PMID | 11083777
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, CD
- Antigens, Differentiation
- B7-1 Antigen
- B7-2 Antigen
- CD28 Antigens
- CD86 protein, human
- CTLA-4 Antigen
- CTLA4 protein, human
- Cd86 protein, rat
- Ctla4 protein, rat
- Immunoconjugates
- Membrane Glycoproteins
- Abatacept
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Topics |
- Abatacept
- Abscess
(etiology)
- Animals
- Antigens, CD
(physiology)
- Antigens, Differentiation
(pharmacology)
- B7-1 Antigen
(physiology)
- B7-2 Antigen
- CD28 Antigens
(physiology)
- CD4-Positive T-Lymphocytes
(immunology)
- CTLA-4 Antigen
- Humans
- Immunoconjugates
- Lymphocyte Activation
- Male
- Membrane Glycoproteins
(physiology)
- Rats
- Rats, Wistar
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