Polyethylene glycol-superoxide dismutase inhibits lipid peroxidation in hepatic ischemia/reperfusion injury.

Abstract

BACKGROUND:

Hepatic injury after ischemia/reperfusion is attributed to the development of oxygen free radical (OFR)-mediated lipid peroxidation--a process that can be measured through its byproducts, specifically malondialdehyde. The use of free radical scavengers can offer significant protection against OFR-induced liver injury. We hypothesize that a new potent OFR scavenger, polyethylene glycol-superoxide dismutase (PEG-SOD), can inhibit OFR-mediated lipid peroxidation in hepatic ischemia/reperfusion injury.

METHODS:

Twelve male Sprague-Dawley rats (300-350 g) were subjected to occlusion of the left and middle hepatic arteries and portal veins for 90 min, followed by 120 min reperfusion. PEG-SOD (5000 units/kg) was given intravenously before vascular occlusion and again immediately upon reperfusion to six rats. Normal saline was given to the remaining six rats to be used as a control group. The right hepatic lobe (used as internal control) and left hepatic lobe were harvested separately and tissue malondialdehyde was measured.

RESULTS:

A marked increase in lipid peroxide was found in the normal saline group after 2 h reperfusion. Treatment with PEG-SOD prevented the rise in tissue malondialdehyde. The mean difference in the malondialdehyde between the left and right hepatic lobes were 13.20 +/- 6.35 and 1.70 +/- 3.65 nmol/g in the normal saline (control) and PEG-SOD groups, respectively. This difference was found to be statistically significant (P < 0.005) using Student's t-test.

CONCLUSIONS:

PEG-SOD can effectively attenuate hepatic ischemia/reperfusion injury by inhibiting OFR-mediated lipid peroxidation.

• Authors: W D Nguyen, D H Kim, H B Alam, H S Provido, J R Kirkpatrick
• Journal: Critical care (London, England) (Crit Care) Vol. 3 Issue 5 Pg. 127-30 ( 1999) ISSN: 1364-8535 [Print] England
• PMID: 11056736 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Free Radical Scavengers
• Polyethylene Glycols
• Malondialdehyde
• Superoxide Dismutase
• polyethylene glycol-superoxide dismutase

Topics

• Animals
• Biomarkers
• Free Radical Scavengers (pharmacology, therapeutic use)
• Lipid Peroxidation (drug effects)
• Liver (injuries, metabolism)
• Male
• Malondialdehyde (metabolism)
• Polyethylene Glycols (pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, prevention & control)
• Superoxide Dismutase (pharmacology, therapeutic use)