Abstract |
This study was carried out to investigate the role of aquaporin (AQP) in the peritoneum undergoing continuous ambulatory peritoneal dialysis ( CAPD). Furthermore, we examined the effects of treatment with prednisolone (PSL) in a rat model of peritoneal sclerosis. We modelled peritoneal sclerosis by using dialysis solution with the addition of 0.1% chlorhexidine gluconate (CHG) for 10 days. Twenty male Wistar Kyoto (WKY) rats were divided into four groups and dialyzed with various solutions: (1) saline (NS group, n = 5); (2) 10% glucose (TZ group, n = 5); (3) 0.1% CHG (CHG group, n = 5); and (4) 0.1% CHG plus PSL (CHG + PSL group, n = 5). Expression of mRNA of AQPs (AQP-1-AQP-4) was studied by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR). Expression of AQP-4 was also measured by Western blot analysis. Ultrafiltration volume and peritoneal function were measured by the peritoneal equilibration test. In the TZ group, expression of AQP-1 and AQP-4 were significantly enhanced, in parallel with an increment in ultrafiltration volume. On the other hand, in the CHG group, expression of AQP-1 and AQP-4 were significantly suppressed, and ultrafiltration volume was lost. The use of PSL with CHG completely restored the expression of AQP-1 and AQP-4, and peritoneal function improved. No expression of AQP-2 and AQP-3 was seen in the peritoneum. Our results suggest that AQP-1 and AQP-4 may be important factors in water transport in patients undergoing CAPD. PSL might be an effective treatment to prevent the progress of peritoneal sclerosis in patients undergoing CAPD.
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Authors | H Imai, H Nakamoto, Y Ishida, T Inoue, Y Kanno, H Okada, S Suzuki, H Okano, H Suzuki |
Journal | Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
(Adv Perit Dial)
Vol. 16
Pg. 297-302
( 2000)
ISSN: 1197-8554 [Print] Canada |
PMID | 11045315
(Publication Type: Journal Article)
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Chemical References |
- Aquaporins
- Dialysis Solutions
- Glucocorticoids
- Sodium Chloride
- Prednisolone
- Glucose
- chlorhexidine gluconate
- Chlorhexidine
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Topics |
- Animals
- Aquaporins
(metabolism)
- Biological Transport
(drug effects)
- Blotting, Western
- Body Water
(metabolism)
- Chlorhexidine
(analogs & derivatives, pharmacology)
- Dialysis Solutions
(pharmacology)
- Glucocorticoids
(pharmacology)
- Glucose
(pharmacology)
- Male
- Peritoneal Dialysis, Continuous Ambulatory
- Peritoneum
(metabolism, pathology)
- Prednisolone
(pharmacology)
- Rats
- Rats, Inbred WKY
- Reverse Transcriptase Polymerase Chain Reaction
- Sclerosis
- Sodium Chloride
(pharmacology)
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