Abstract |
We studied the effects of diaspirin cross-linked hemoglobin ( DCLHb), a cell-free hemoglobin derived from human erythrocytes, on blood flow distribution and tissue oxygen extraction capabilities in endotoxic shock. Eighteen pentobarbital sodium-anesthetized, mechanically ventilated dogs received 2 mg/kg of E. coli endotoxin, followed by saline resuscitation to restore cardiac filling pressures to baseline levels. The animals were randomly divided into three groups: six served as control, six received DCLHb at a dose of 500 mg/kg (group 1) and six DCLHb at a dose of 1,000 mg/kg (group 2). Cardiac tamponade was then induced by saline injection in the pericardial sac to progressively reduce cardiac index and thereby allow study of tissue oxygen extraction capabilities. DCLHb had a dose-dependent vasopressor effect but did not significantly alter cardiac index or regional blood flow. During cardiac tamponade, critical oxygen delivery was 12.8 +/- 0.7 ml. kg(-1). min(-1) in the control group, but 8.6 +/- 0.9 and 8.2 +/- 0.7 ml. kg(-1). min(-1) in groups 1 and 2, respectively (both P < 0.05 vs. control group). The critical oxygen extraction ratio was 39.1 +/- 3.1% in the control group but 58.7 +/- 12.8% and 60.2 +/- 9.0% in groups 1 and 2, respectively. We conclude that DCLHb can improve whole body oxygen extraction capabilities during endotoxic shock in dogs.
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Authors | J Creteur, H Zhang, D De Backer, Q Sun, J L Vincent |
Journal | Journal of applied physiology (Bethesda, Md. : 1985)
(J Appl Physiol (1985))
Vol. 89
Issue 4
Pg. 1437-44
(Oct 2000)
ISSN: 8750-7587 [Print] United States |
PMID | 11007580
(Publication Type: Journal Article)
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Chemical References |
- Blood Substitutes
- Endotoxins
- Hemoglobins
- diaspirin-cross-linked hemoglobin
- Aspirin
- Oxygen
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Topics |
- Animals
- Aspirin
(analogs & derivatives, pharmacology)
- Blood Pressure
(drug effects)
- Blood Substitutes
(pharmacology)
- Cardiac Output
(drug effects)
- Cardiac Tamponade
- Dogs
- Endotoxins
- Escherichia coli
- Hemodynamics
(drug effects, physiology)
- Hemoglobins
(pharmacology)
- Oxygen
(blood)
- Oxygen Consumption
(drug effects)
- Regional Blood Flow
(drug effects)
- Shock, Septic
(blood, physiopathology, therapy)
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