A TVA-single-chain antibody fusion protein mediates specific targeting of a subgroup A avian leukosis virus vector to cells expressing a tumor-specific form of epidermal growth factor receptor.

Abstract

We have previously described an approach that employs retroviral receptor-ligand bridge proteins to target retroviral vectors to specific cell types. To determine whether targeted retroviral entry can also be achieved using a retroviral receptor-single-chain antibody bridge protein, the TVA-MR1 fusion protein was generated. TVA-MR1 is comprised of the extracellular domain of the TVA receptor for subgroup A avian leukosis viruses (ALV-A), fused to the MR1 single-chain antibody that binds specifically to EGFRvIII, a tumor-specific form of the epidermal growth factor receptor. We show that TVA-MR1 binds specifically to a murine version of EGFRvIII and promotes ALV-A entry selectively into cells that express this cell surface marker. These studies demonstrate that it is possible to target retroviral vectors to specific cell types through the use of a retroviral receptor-single-chain antibody fusion protein.

• Authors: S Snitkovsky, T M Niederman, B S Carter, R C Mulligan, J A Young
• Journal: Journal of virology (J Virol) Vol. 74 Issue 20 Pg. 9540-5 (Oct 2000) ISSN: 0022-538X [Print] United States
• PMID: 11000224 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Avian Proteins
• Histocompatibility Antigens Class I
• MR1 protein, human
• Minor Histocompatibility Antigens
• Receptors, Virus
• Recombinant Fusion Proteins
• Tva receptor
• ErbB Receptors

Topics

• Avian Leukosis Virus (physiology)
• Avian Proteins
• Cell Line
• ErbB Receptors (metabolism)
• Genetic Vectors
• Histocompatibility Antigens Class I (metabolism)
• Humans
• Minor Histocompatibility Antigens
• Receptors, Virus (metabolism)
• Recombinant Fusion Proteins (metabolism)