Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils.
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| Abstract |
Interleukin-9 (IL-9) has been implicated in the pathogenesis of allergic disorders. To examine the interaction between IL-9 and eosinophils, we evaluated mature peripheral blood eosinophils for their expression of the specific alpha-subunit of the IL-9 receptor (IL-9R-alpha). The expression of IL-9R-alpha by human eosinophils was detected at the messenger RNA (mRNA) and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, and immunocytochemical analysis, respectively. Functional analyses demonstrated that recombinant human (rh)IL-9 inhibited in vitro peripheral blood human eosinophil apoptosis in a concentration-dependent manner. We then examined the role of IL-9 in eosinophil differentiation using the human cord blood CD34(+) cells and human promyelocytic leukemia cells (HL-60). The addition of IL-9 to CD34(+) cells cultured in IL-3 and IL-5 enhanced eosinophil development, and IL-9 alone induced the expression of IL-5R-alpha. IL-9 also up-regulated the IL-5R-alpha chain cell surface expression during terminal eosinophil differentiation of the HL-60 cell line. Our findings suggest that IL-9 may potentiate in vivo eosinophil function by increasing their survival and IL-5-mediated differentiation and maturation. Taken together, these results suggest a mechanism by which IL-9 potentiates airway and tissue eosinophilia.
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| Authors | A S Gounni, B Gregory, E Nutku, F Aris, K Latifa, E Minshall, J North, J Tavernier, R Levit, N Nicolaides, D Robinson, Q Hamid |
| Journal | Blood (Blood) Vol. 96 Issue 6 Pg. 2163-71 (Sep 15 2000) ISSN: 0006-4971 [Print] United States |
| PMID | 10979962 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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