The aim of this study was evaluation of
DNA ploidy in the cytological samples from patients with
laryngeal cancer and comparison a type of
DNA ploidy with histological grading (G) and staging of neoplasmatic process. The examinations were performed on 47 patients operated due to
laryngeal cancer (3 women, 44 men, mean age 58). Cytological material was collected from
squamous carcinoma tissue by imprinting method. Slides were staining by Feulgen method in order to quantitative analysis of
DNA by static cytophotometry. In studied material,
aneuploid,
polyploid and hypoploid
tumors were found in 22 patients and diploid
tumors were found in 25 cases. Analysis of
DNA ploidy type distribution in correlation to G feature showed:--diploid type were dominated in G1 and G2
tumors,--
aneuploid and
polyploid type were dominated in G3
tumors. Hypoploid and
polyploid tumors were appeared in patients with
metastases to cervical lymph nodes more frequently than in patients without
metastases. Analysis of
DNA ploidy complete traditional histopathological diagnosis of
laryngeal cancer and may be useful in predicting
metastases to cervical lymph nodes.