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5-aminolevulinic acid induced lipid peroxidation after light exposure on human colon carcinoma cells and effects of alpha-tocopherol treatment.

Abstract
This work relates to studies on modes of phototoxicity by protoporphyrin (PpIX) after incubation of 5-aminolevulinic acid (5-ALA) on cultured cells. Lipid peroxidation in the 5-ALA incubated primary adenocarcinoma cells from the rectosigmoid colon (WiDr cells) was determined by measurement of protein-associated thiobarbituric acid reactive substances (TBARS). TBARS were increased 2-fold in cells treated with 2 mM 5-ALA for 3.5 h in serum enriched medium. After illumination of 5-ALA incubated cells, TBARS were formed in a light dose dependent manner. TBARS analysis were compared with high-performance liquid chromatography (HPLC) analysis of malondialdehyde, and results indicate that 90% of the thiobarbituric reactive substances were due to malondialdehyde. Pretreating WiDr cells with alpha-tocopherol for 48 h inhibits the cytotoxic effect of 5-ALA and increases 5-fold the light dose needed to kill 50% of the cells. Pretreatment with alpha-tocopherol shows a considerable decrease (about 80%) on TBARS formation after illumination. The cellular content of alpha-tocopherol was determined by HPLC and found to be 15.3 pmol/10(6) cells.
AuthorsO A Gederaas, J W Lagerberg, O Brekke, K Berg, T M Dubbelman
JournalCancer letters (Cancer Lett) Vol. 159 Issue 1 Pg. 23-32 (Oct 16 2000) ISSN: 0304-3835 [Print] Ireland
PMID10974402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Photosensitizing Agents
  • Thiobarbituric Acid Reactive Substances
  • Vitamin E
  • Malondialdehyde
  • Aminolevulinic Acid
Topics
  • Adenocarcinoma (metabolism, pathology, prevention & control)
  • Aminolevulinic Acid (pharmacology)
  • Cell Survival (drug effects, radiation effects)
  • Colonic Neoplasms (metabolism, pathology, prevention & control)
  • Dose-Response Relationship, Radiation
  • Humans
  • Light
  • Lipid Peroxidation (drug effects)
  • Malondialdehyde (metabolism, radiation effects)
  • Photosensitizing Agents (pharmacology)
  • Thiobarbituric Acid Reactive Substances (metabolism, radiation effects)
  • Tumor Cells, Cultured (drug effects, metabolism, radiation effects)
  • Vitamin E (pharmacokinetics, pharmacology)

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