Virus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands.

Abstract

Bis-(8-anilinonaphthalene-1-sulfonate) (bis-ANS) causes inactivation of vesicular stomatitis virus (VSV) at micromolar concentrations while butyl-ANS and ANS are effective at concentrations one and two orders of magnitude higher, respectively. VSV fully inactivated by the combined effects of 10 microM bis-ANS and 2.5 kbar hydrostatic pressure elicited a high titer of neutralizing antibodies. Incubation of VSV with >/=2 M urea at atmospheric pressure caused very little virus inactivation, whereas at a pressure of 2.5 kbar, 1 M urea caused inactivation that exceeded by more than two orders of magnitude the sum of the inactivating effects produced by urea and pressure separately. Measurements of bis-ANS fluorescence showed that increasing the urea concentration reduces the pressure required to disrupt the structure. We conclude that anilinonaphthalene sulfonate compounds inactivate VSV by a mechanism similar to that produced by pressure. The most effective antiviral compound was bis-ANS which can be used for the preparation of safe viral vaccines or as an antiviral drug eventually.

• Authors: C F Bonafe, M Glaser, E W Voss, G Weber, J L Silva
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 275 Issue 3 Pg. 955-61 (Sep 07 2000) ISSN: 0006-291X [Print] United States
• PMID: 10973827 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Anilino Naphthalenesulfonates
• Antibodies
• Antiviral Agents
• Serum Albumin
• 1-anilino-8-naphthalenesulfonate
• 5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate)
• Urea
• Xylose

Topics

• Anilino Naphthalenesulfonates (chemistry, metabolism, pharmacology, therapeutic use)
• Animals
• Antibodies (pharmacology)
• Antiviral Agents (chemistry, metabolism, pharmacology, therapeutic use)
• Cell Line
• Cricetinae
• Dose-Response Relationship, Drug
• Fluorescence
• Hydrostatic Pressure
• Serum Albumin (pharmacology)
• Urea (pharmacology)
• Vesicular stomatitis Indiana virus (drug effects, immunology, physiology)
• Xylose (pharmacology)