Abstract | BACKGROUND: OBJECTIVE: To characterize tissue-factor-mediated generation of thrombin and its association with tissue injury during reperfusion from normothermic ischemia of isolated, Langendorf-perfused rabbit hearts. METHODS: RESULTS: Concentrations of hFPA in the effluent were minimal during non-ischemic perfusion (5 +/- 5 ng/ml, n=6) and during 50 min of ischemia (13 +/- 3 ng/ml, n=6), but increased markedly during the first 20 min of reperfusion (to 41 +/- 29 ng/ml, P=0.03 versus before reperfusion). Addition to the perfusate of 10 microg/ml recombinant human tissue-factor-pathway inhibitor, the physiologic inhibitor of tissue-factor-mediated coagulation, abolished increases in the level of hFPA after reperfusion. However, indexes of myocardial injury manifested during reperfusion, including decrease in recovery of left ventricular pressure developed, increase in left ventricular end-diastolic pressure, and increase in activity of creatine kinase in the heart effluent, were not improved by anticoagulation with recombinant human tissue-factor-pathway inhibitor. CONCLUSION: Our results do not support the hypothesis that coagulation plays a major role in ischemia/reperfusion injury of Langendorf-perfused rabbit hearts.
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Authors | L Armaganian, G Kam, P R Eisenberg, K B Schechtman, D R Abendschein |
Journal | Coronary artery disease
(Coron Artery Dis)
Vol. 11
Issue 6
Pg. 481-7
(Sep 2000)
ISSN: 0954-6928 [Print] England |
PMID | 10966134
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fibrinolytic Agents
- Lipoproteins
- Peptide Fragments
- tissue factor pathway inhibitor (1-161)
- Fibrinopeptide A
- Thromboplastin
- Creatine Kinase
- Thrombin
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Topics |
- Animals
- Blood Coagulation
(drug effects, physiology)
- Coronary Vessels
(physiology)
- Creatine Kinase
(blood)
- Fibrinolytic Agents
(pharmacology, therapeutic use)
- Fibrinopeptide A
(analysis)
- Heart
(physiology)
- Humans
- Lipoproteins
(pharmacology, therapeutic use)
- Peptide Fragments
(pharmacology, therapeutic use)
- Rabbits
- Reperfusion Injury
(drug therapy, metabolism, physiopathology)
- Thrombin
(biosynthesis)
- Thromboplastin
(antagonists & inhibitors, physiology)
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