Abstract |
Genistein, a prominent isoflavone in soy products, produced dose- and time-dependent in vitro growth inhibition at high concentrations (at least 185 microM) with an IC50 of 7.0-274.2 microM after 72 h incubation in four breast cancer cell lines (DD-762, Sm-MT, MCF-7 and MDA-MB-231) and one breast epithelial cell line (HBL- 100) of human and animal origin; it stimulated estrogen-receptor-positive MCF-7 cells at low concentrations (3.7 nM-37 microM). Genistein-exposed cells underwent apoptosis, confirmed by G2/M arrest followed by the appearance of a sub-G1 fraction in cell-cycle progression, and by a characteristic cell ultrastructure. The apoptosis cascade was due to up-regulation of Bax protein, down-regulation of Bcl-XL protein, and activation of caspase-3. Genistein acted in synergism with eicosapentaenoic acid (EPA), a fish oil component, on human breast cancer MCF-7 cells ( genistein > 93.2 microM and EPA > 210.9 microM) and on MDA-MB-231 cells ( genistein > 176.1 microM and EPA > 609.3 microM). Dietary intake of genistein in combination with EPA may be beneficial for breast cancer control.
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Authors | H Nakagawa, D Yamamoto, Y Kiyozuka, K Tsuta, Y Uemura, K Hioki, Y Tsutsui, A Tsubura |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 126
Issue 8
Pg. 448-54
(Aug 2000)
ISSN: 0171-5216 [Print] Germany |
PMID | 10961387
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Growth Inhibitors
- Receptors, Estrogen
- Eicosapentaenoic Acid
- Genistein
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Topics |
- Animals
- Apoptosis
- Breast Neoplasms
(metabolism, pathology, prevention & control)
- Cell Cycle
(drug effects)
- Diet
- Dose-Response Relationship, Drug
- Drug Synergism
- Eicosapentaenoic Acid
(pharmacology)
- Eulipotyphla
- Female
- Flow Cytometry
- Genistein
(pharmacology)
- Growth Inhibitors
(pharmacology)
- Humans
- Mice
- Microscopy, Electron
- Receptors, Estrogen
- Tumor Cells, Cultured
(drug effects)
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