Preventive effects of danazol on endometrial carcinogenesis in mice.

Abstract

Short and long-term experiments were designed to determine effects of danazol on estrogen-related endometrial carcinogenesis in mice. The short-term assays showed that danazol decreased expression levels of c-fos/jun mRNA and their oncoproteins induced by estradiol-17beta (E2). For the long-term assay, 85 female ICR mice were given N-methyl-N-nitrsourea solution into their uterine corpora. The animals were divided into three groups as follows: Group 1, E2-diet (5 ppm) plus danazol (2 mg/body (s.c.), every 4 weeks); Group 2, E2-diet alone, Group 3, basal diet alone. At 30 weeks, incidences of atypical and complex endometrial hyperplasia were significantly decreased by danazol-treatment. These results suggest that danazol has preventive effects on estrogen-related endometrial carcinogenesis in mice, through the suppression of estrogen-induced c-fos/jun-expression.

• Authors: K Niwa, M Hashimoto, S Morishita, Y Yokoyama, Z Lian, K Tagami, H Mori, T Tamaya
• Journal: Cancer letters (Cancer Lett) Vol. 158 Issue 2 Pg. 133-9 (Oct 01 2000) ISSN: 0304-3835 [Print] Ireland
• PMID: 10960762 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkylating Agents
• Estrogen Antagonists
• Proliferating Cell Nuclear Antigen
• Proto-Oncogene Proteins c-fos
• Proto-Oncogene Proteins c-jun
• RNA, Messenger
• Estradiol
• Methylnitrosourea
• Danazol

Topics

• Administration, Oral
• Alkylating Agents (toxicity)
• Animals
• Danazol (therapeutic use)
• Endometrial Neoplasms (chemically induced, metabolism, prevention & control)
• Estradiol (pharmacology)
• Estrogen Antagonists (therapeutic use)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Immunohistochemistry
• Methylnitrosourea (toxicity)
• Mice
• Mice, Inbred ICR
• Proliferating Cell Nuclear Antigen (analysis)
• Proto-Oncogene Proteins c-fos (analysis, genetics)
• Proto-Oncogene Proteins c-jun (analysis, genetics)
• RNA, Messenger (drug effects, genetics, metabolism)
• Uterus (chemistry, pathology)