Neutrophil elastase (NE) is the only neutral
protease that can degrade broad substrates of extracellular matrix components. In the present study, we describe the NE-like molecule expressed in many
carcinoma cells, which has similar activity to NE and
pancreatic elastase, but is immunologically different from NE, and is not inhibited by NE or
pancreatic elastase inhibitor at all. Intracellular activity of NE or
pancreatic elastase and immunological reactivity of NE in ten
carcinoma cell lines and freshly purified neutrophils were measured by CellProbe and
enzyme immunoassay, respectively. The NE and pancreatic inhibitory effect to the extracts of the ten cell lines was further examined using NE and
pancreatic elastase inhibitor (ONO-5046.Na). Only two
carcinoma cell lines out of ten had low immunoreactive NE, whereas neutrophils had high immunoreactivity in the extract. Flow cytometric analyses demonstrated that five out of 11
carcinoma cell lines had a high degrading activity of
Ala-Ala-Pro-Val site in more than half of the population. SUIT-2 had the highest activity, but had no immunoreactivity for NE. Furthermore, the NE-like activity in the SUIT-2 cells was not inhibited by ONO-5046.Na. The present study demonstrated the NE-like molecule expressed in many types of
carcinoma cells, which is potentially a new and specific
protease produced by
cancer cells. It would be of great interest to identify this NE-like molecule specific to the
tumor, leading to a possible promising treatment of advanced
carcinomas.