Induction of Th1 response by dendritic cells pulsed with autologous melanoma apoptotic bodies.

Abstract

BACKGROUND:

We hypothesize that dendritic cells (DCs) can process antigens from autologous melanoma apoptotic bodies (MABs) and induce effector T cells in melanoma patients.

MATERIALS AND METHODS:

Peripheral blood mononuclear cells were obtained from three stage IV melanoma patients and adherent cells were cultured in complete medium (CM) containing GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 7 days. Autologous MABs from melanoma cells following actinomycin D treatment (0.5 microgram/ml) for 24 hours, were added to 72 hour DC culture. Autologous effector T cells were cultured in CM containing 60 IU/ml of IL-2 and were stimulated by MAB-pulsed DCs three times at a weekly interval. Effector T cells were harvested at the end of third cycle of DC stimulation.

RESULTS:

Using ELISPOT, IFN-gamma production by effector T cells stimulated by MAB-pulsed DCs was significantly higher than that by T cells without DC stimulation. Microscopy demonstrated phagocytosis of MABs by DCs.

CONCLUSIONS:

MAB-pulsed DCs are capable of stimulating Th1-directed autologous effector T cells. Pulsing DCs with autologous MABs may be a novel approach in future DC-based immunotherapeutic trials.

• Authors: J W Chang, M Peng, J E Vaquerano, Y M Zhou, R A Clinton, W C Hyun, M A Giedlin, S P Leong
• Journal: Anticancer research (Anticancer Res) 2000 May-Jun Vol. 20 Issue 3A Pg. 1329-36 ISSN: 0250-7005 [Print] Greece
• PMID: 10928040 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Interleukin-10
• Interferon-gamma

Topics

• Antigens, Neoplasm (immunology)
• Apoptosis (immunology)
• Dendritic Cells (immunology, physiology)
• Humans
• In Vitro Techniques
• Interferon-gamma (analysis)
• Interleukin-10 (analysis)
• Lymphocyte Activation (immunology)
• Melanoma (pathology)
• Phagocytosis
• Phenotype
• T-Lymphocytes (immunology)
• Th1 Cells (immunology)