Abstract | OBJECTIVE: MATERIALS AND METHODS: Young adult Rhesus monkeys were subjected to 8-Gy total body irradiation (TBI) (x-rays) followed by transplantation of 10(7)/kg unfractionated bone marrow cells. TPO 5 microg/kg was administered intravenously at day 0 to obtain rapidly high levels. Animals then were treated with 5 microg/kg Rhesus TPO and 25 microg/kg GM-CSF given SC on days 1 to 14 after TBI. RESULTS: The grafts shortened the profound pancytopenia induced by 8-Gy TBI from 5-6 weeks to 3 weeks. The combination of TPO and GM-CSF did not significantly influence the recovery patterns of thrombocytes (p = 0.39), reticulocytes (p = 0.08), white blood cells (p = 0.08), or bone marrow progenitors compared to TPO alone. CONCLUSIONS: The present study demonstrates that, after high-dose TBI and transplantation of a limited number of unfractionated bone marrow cells, simultaneous administration of TPO and GM-CSF after TBI is ineffective in preventing pancytopenia. This result contrasts sharply with the prominent stimulation observed in a 5-Gy TBI myelosuppression model, despite a similar level of pancytopenia in the 8-Gy model of the present study. The discordant results of this growth factor combination in these two models may imply codependence of the hematopoietic response to TPO and/or GM-CSF on other factors or cytokines.
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Authors | S C Hartong, K J Neelis, T P Visser, G Wagemaker |
Journal | Experimental hematology
(Exp Hematol)
Vol. 28
Issue 7
Pg. 753-9
(Jul 2000)
ISSN: 0301-472X [Print] Netherlands |
PMID | 10907636
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Granulocyte-Macrophage Colony-Stimulating Factor
- Thrombopoietin
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Topics |
- Animals
- Bone Marrow Transplantation
- Cell Separation
- Drug Therapy, Combination
- Flow Cytometry
- Granulocyte-Macrophage Colony-Stimulating Factor
(administration & dosage, therapeutic use)
- Lymphocyte Subsets
(cytology)
- Macaca mulatta
- Male
- Thrombopoietin
(administration & dosage, therapeutic use)
- Whole-Body Irradiation
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