Abstract |
A coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of rat liver carbonic anhydrase III (CAIII), an enzyme which has recently been suggested to prevent from H(2)O(2)-inducible apoptosis. Marked changes in the CAIII levels of liver cytosol were observed in rats following doses of PenCB ranging from 0.5 to 25 mg/kg body weight and maximum suppression was observed at a dose of 10 mg/kg. Northern analysis revealed that the level of CAIII mRNA in rat liver was dramatically reduced by PenCB treatment while only weak suppression was observed in pair-fed controls. Two AU-rich elements, considered as a destabilizing signal of mRNA, were found in the 3'-untranslated region of CAIII sequenced after reverse transcription-PCR and 3'-rapid amplification of the cDNA end. Dramatic decrease of CAIII in rat liver by PenCB could account for the suppression of the defense system for oxidative stress.
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Authors | M Ikeda, Y Ishii, H Kato, D Akazawa, M Hatsumura, T Ishida, K Matsusue, H Yamada, K Oguri |
Journal | Archives of biochemistry and biophysics
(Arch Biochem Biophys)
Vol. 380
Issue 1
Pg. 159-64
(Aug 01 2000)
ISSN: 0003-9861 [Print] United States |
PMID | 10900145
(Publication Type: Journal Article)
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Copyright | Copyright 2000 Academic Press. |
Chemical References |
- 3' Untranslated Regions
- Carbonic Anhydrase Inhibitors
- DNA, Complementary
- Polychlorinated Biphenyls
- Protein Tyrosine Phosphatases
- Carbonic Anhydrases
- 3,4,5,3',4'-pentachlorobiphenyl
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Topics |
- 3' Untranslated Regions
- Animals
- Blotting, Northern
- Carbonic Anhydrase Inhibitors
(pharmacology)
- Carbonic Anhydrases
(genetics, metabolism)
- Cloning, Molecular
- Cytosol
(enzymology)
- DNA, Complementary
(metabolism)
- Dose-Response Relationship, Drug
- Immunoblotting
- Liver
(enzymology)
- Male
- Molecular Sequence Data
- Oxidation-Reduction
(drug effects)
- Oxidative Stress
- Polychlorinated Biphenyls
(pharmacology)
- Protein Tyrosine Phosphatases
(metabolism)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
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