Abstract |
PECAM-1 is a 130-kd member of the Ig superfamily present on endothelial cells, platelets, polymorphonuclear leukocytes, monocytes, and lymphocytes. Its expression begins early in development and persists through adulthood. PECAM-1 functions as an adhesion and signaling molecule between adjacent endothelial cells and between endothelial cells and circulating blood elements. Antibodies directed against PECAM-1 have been shown to affect angiogenesis, endothelial cell migration, and polymorphonuclear leukocyte transmigration. Furthermore, its dimerization is associated with the modulation of integrin affinity. Antibody inhibition studies suggest that PECAM-1 plays a role in modulating thrombosis; however, recent in vitro aggregation studies performed on platelets harvested from PECAM-1-deficient mice revealed no abnormalities. In this report we demonstrate prolonged in vivo bleeding times in PECAM-1-deficient mice. This abnormality was not corrected when wild-type hematopoietic precursors were engrafted into marrow-ablated PECAM-1-deficient mice. Furthermore, normal bleeding times were observed when marrow-ablated wild-type mice were engrafted with hematopoietic precursors harvested from PECAM-1-deficient mice. These studies are consistent with a role for PECAM-1 in modulating thrombosis in the vasculature, which is potentially mediated by endothelial cell PECAM-1 expression.
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Authors | S Mahooti, D Graesser, S Patil, P Newman, G Duncan, T Mak, J A Madri |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 157
Issue 1
Pg. 75-81
(Jul 2000)
ISSN: 0002-9440 [Print] United States |
PMID | 10880378
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Platelet Endothelial Cell Adhesion Molecule-1
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Topics |
- Animals
- Bleeding Time
- Female
- Hematopoietic Stem Cell Transplantation
- Hematopoietic Stem Cells
(cytology, metabolism)
- Heterozygote
- Homozygote
- Male
- Mice
- Mice, Knockout
- Platelet Count
- Platelet Endothelial Cell Adhesion Molecule-1
(biosynthesis, genetics)
- Time Factors
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