Intracerebral
infection of susceptible strains of mice with Theiler's murine encephalomyelitis virus (TMEV) induces a biphasic disease characterized by acute polioencephalitis followed by chronic
demyelination and viral persistence in the spinal cord white matter. There has been limited study of soluble mediators responsible for the initial recruitment of inflammatory cells into the gray matter, and the secondary influx into the white matter during
infection with TMEV. We used sensitive and specific RT - PCR/dot blot hybridization assays to quantitate the relative levels of
chemokine mRNA in the brains and spinal cords during the acute and chronic phases of TMEV
infection in mice susceptible (B10.M, H-2f) and resistant (B10, H-2b) to virus-induced
demyelination. TMEV
infection resulted in robust expression of
mRNA for IP-10,
RANTES, and MCP-1, but not GRO-alpha, in brains and spinal cords in both strains of mice within 5 days. By day 21, virus was cleared,
inflammation reduced, and expression of all three
chemokines subsided to baseline levels in the brains and spinal cords of resistant mice, and the brains of susceptible mice.
Chemokine expression was also reduced in the spinal cords of susceptible mice, corresponding to a shift in TMEV replication from the gray to the white matter. During the chronic, demyelinating phase of
infection, there was a resurgence in IP-10,
RANTES, and MCP-1
mRNA in spinal cords of susceptible B10.M mice. This study demonstrates the coordinated regulation and regionally restricted expression of
chemokines in a biphasic disease of the central nervous system and provides greater understanding of the mechanism by which
inflammation is established and maintained in the CNS.