Abstract |
In this study, we hypothesize that anti- cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine CK18 was stained with patients' sera using a Western blotting. In patients with IPF, anti-CK18 antibodies were clearly demonstrated in sera by Western blotting. Then, we tried to establish an enzyme-linked immunosorbent assay (ELISA) to quantify anti-CK18 antibodies and CK18:anti-CK18 immune complexes in sera of patients with IPF. Levels of anti-human CK18 antibodies in sera of patients with IPF (0.81 +/- 0.31, mean +/- SD) measured by ELISA were significantly high compared with that of normal volunteers (0.45 +/- 0.06, p < 0.01). In addition, levels of CK18:anti-CK18 antibody complexes in patients' sera (0.64 +/- 0.35, man +/- SD) significantly increased compared with those of normal subjects (0.40 +/- 0.10, p < 0.01). These results suggest that anti-CK18 antibody and its immune complex may have played a role in the process of lung injury in IPF.
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Authors | N Dobashi, J Fujita, M Murota, Y Ohtsuki, I Yamadori, T Yoshinouchi, R Ueda, S Bandoh, T Kamei, M Nishioka, T Ishida, J Takahara |
Journal | Lung
(Lung)
Vol. 178
Issue 3
Pg. 171-9
( 2000)
ISSN: 0341-2040 [Print] United States |
PMID | 10871435
(Publication Type: Comparative Study, Journal Article, Multicenter Study)
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Chemical References |
- Antigen-Antibody Complex
- Autoantibodies
- Biomarkers
- Keratins
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Topics |
- Adult
- Aged
- Antigen-Antibody Complex
(blood, immunology)
- Autoantibodies
- Biomarkers
(blood)
- Blotting, Western
- Cell Division
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Keratins
(immunology)
- Male
- Middle Aged
- Prognosis
- Pulmonary Alveoli
(immunology, pathology)
- Pulmonary Fibrosis
(blood, immunology, pathology)
- Retrospective Studies
- Severity of Illness Index
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