[Expression in vivo of the lacZ gene, delivered to mouse lungs using synthetic microspheres].
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| Abstract |
A capacity of MF-2 synthetic microspheres to serve as the vehicle for transfer of the marker LacZ gene to mouse lung epithelial cells was studied after a single intranasal administration of the MF-2/gene complex. Two types of plasmids carrying marker gene LacZ were used in the experiments: with cytoplasmic (pCMV-LacZ) and nuclear (pCMV-nlsLacZ) localization of the gene product (beta-galactosidase). As early as 7 days after the complexes MF-2/pCMV-LacZ and MF-2/pCMV-nlsLacZ were administered, specific staining for beta-galactosidase revealed this enzyme activity in the epithelial cells of bronchi, bronchioli, and alveoli. The maximum in vivo of the marker gene in the MF-2/pCMV-LacZ complex was observed at day 14 to 21 after administration and the corresponding gene product was detected during the following two months. The MF-2-mediated gene transfer led to a twofold increase in beta-galactosidase activity relative to the case when the "unbound" pCMV-LacZ plasmid was administered. These results suggest that the synthetic microsphere-mediated transfer of alien genes to the lung of experimental animals is promising. Microspheres may be used in gene therapy for pulmonary affections, in particular cystic fibrosis.
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| Authors | P B Glazkov, T E Ivashchenko, V A Sabetskiĭ, B Scholte, BaranovVS |
| Journal | Genetika (Genetika) Vol. 36 Issue 5 Pg. 606-12 (May 2000) ISSN: 0016-6758 [Print] Russia (Federation) |
| Vernacular Title | Ekspressiia in vivo gena lacZ, dostavlennogo v legkie mysheĭ pri pomoshchi sinteticheskikh mikrosfer. |
| PMID | 10867875 (Publication Type: English Abstract, Journal Article) |
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