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Distribution and characterization of GFP(+) donor hematogenous cells in Twitcher mice after bone marrow transplantation.

Abstract
The twitcher mouse is a murine model of globoid cell leukodystropy, a genetic demyelinating disease caused by a mutation of the galactosylceramidase gene. Demyelination of the central nervous system commences around 20 postnatal days. Using GFP-transgenic mice as donors, the distribution of hematogenous cells after bone marrow transplantation was investigated in the twitcher mice. Bone marrow transplantation was carried out at 8 postnatal days. In twitcher chimeric mice examined before 30 postnatal days, numerous GFP(+) cells were detected in spleen and peripheral nerve but only a few were detected in the liver, lung, and spinal white matter. In contrast, at 35 to 40 postnatal days when demyelination is evident, many GFP(+) cells with ameboid form were detected in the white matter of the spinal cord, brainstem, and cerebrum. Approximately half of these GFP(+) cells were co-labeled with Mac-1. In twitcher chimeric mice examined after 100 postnatal days, the majority of GFP/Mac-1 double-positive cells displayed the morphological features of ramified microglia with fine delicate processes and was distributed diffusely in both gray and white matter. These results suggest that a significant number of donor hematogenous cells are able to infiltrate into the brain parenchyma, repositioning themselves into areas previously occupied by microglia, and to ameliorate lethality.
AuthorsY P Wu, E McMahon, M R Kraine, R Tisch, A Meyers, J Frelinger, G K Matsushima, K Suzuki
JournalThe American journal of pathology (Am J Pathol) Vol. 156 Issue 6 Pg. 1849-54 (Jun 2000) ISSN: 0002-9440 [Print] United States
PMID10854208 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Indicators and Reagents
  • Luminescent Proteins
  • Green Fluorescent Proteins
Topics
  • Animals
  • Blood Cells (metabolism, transplantation)
  • Bone Marrow Transplantation
  • Central Nervous System (metabolism)
  • Green Fluorescent Proteins
  • Indicators and Reagents
  • Luminescent Proteins (blood, genetics, metabolism)
  • Mice
  • Mice, Inbred C57BL
  • Mice, Neurologic Mutants
  • Mice, Transgenic
  • Peripheral Nerves (metabolism)
  • Postoperative Care
  • Reference Values
  • Tissue Distribution
  • Tissue Donors
  • Viscera (metabolism)

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