High-molecular-weight serum protein complexes differentially promote cell migration and the focal adhesion localization of the urokinase receptor in human glioma cells.

Abstract

The distribution of the urokinase-type plasminogen activator receptor (uPAR) on human glioma cells was examined as a function of culture conditions, using immunofluorescence and immunophotoelectron microscopy. Both uPAR colocalization with focal adhesion proteins and glioma cell motility were maximal in medium containing whole serum or a serum fraction retained by a 500,000 mol wt cutoff centrifugal concentration filter. High motility also took place in medium containing a serum fraction passed by the 500,000 cutoff filter but retained by a 100,000 cutoff filter and in minimal medium containing added vitronectin; however, under these conditions only a small percentage of the otherwise abundant focal adhesions contained colocalized uPAR. Glioma cells in minimal medium with added laminin migrated with a highly elongated morphology but without either classical focal adhesions or well-defined uPAR labeling. In contrast, glioma cells in minimal medium with no additions did not migrate, nor did they adhere well or display defined labeling patterns for focal adhesion proteins or uPAR. The results indicate that high-molecular-weight serum protein complexes promote both uPAR-focal adhesion colocalization and cell migration in glioma cells. However, conditions can be selected in which migration takes place with minimal uPAR-focal adhesion localization, as well as in the absence of apparent focal adhesions.

• Authors: K K Hedberg, C Stauff, G Høyer-Hansen, E Rønne, O H Griffith
• Journal: Experimental cell research (Exp Cell Res) Vol. 257 Issue 1 Pg. 67-81 (May 25 2000) ISSN: 0014-4827 [Print] United States
• PMID: 10854055 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Laminin
• PLAUR protein, human
• Receptors, Cell Surface
• Receptors, Urokinase Plasminogen Activator
• Vitronectin
• Urokinase-Type Plasminogen Activator

Topics

• Cell Adhesion
• Cell Movement
• Glioma (metabolism, pathology)
• Humans
• Intercellular Junctions
• Laminin (metabolism)
• Receptors, Cell Surface (metabolism)
• Receptors, Urokinase Plasminogen Activator
• Tumor Cells, Cultured
• Urokinase-Type Plasminogen Activator (metabolism)
• Vitronectin (metabolism)